γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium munyu Cas: 63699-78-5 Chiedza chero microcrystalline poda
| Catalog Number | XD90183 |
| Product Name | γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium munyu |
| CAS | 63699-78-5 |
| Molecular Formula | C12H12N3O7-.H4N+ |
| Molecular Weight | 328.27 |
| Storage Details | Ambient |
Chigadzirwa Specification
| Chitarisiko | Chiedza chero microcrystalline poda |
| Assay | 99% |
| Melting point | 187ºC ± 2.0ºC |
| Boiling point | 718.3°C pa760 mmHg |
| Flash point | 368.5 °C |
| Solubility | H2O: 100 mg/mL, yakajeka, yero-girinhi |
Iyo kinetics yemunhu serum gamma-glutamyltransferase (EC 2.3.2.2) yakaongororwa, nekushandiswa kweglycylglycine segamma-glutamyl inobvuma substrate uye gamma-glutamyl-4-nitroanilide uye iyo carboxy derivative, gamma-glutamyl-3-carboxy-4 -nitroanilide, sevanopa substrates.Kuitika panguva imwe chete kwezvose zviri zviviri gamma-glutamyltransfer uye autotransfer zvakasimbiswa nekudzika bepa chromatography.Constant-ratio double-reciprocal plots inosimbisa kuti enzyme mechanism haina kukosha (ping-pong bi-bi).Kudzivirirwa nemumwe mupi hakuna kuwanikwa, uye kudziviswa neglycylglycine kwaingoonekwa pane zvakaiswa pamusoro peaya ekiriniki kufarira.Kinetic constants yakawanikwa nenonlinear regression analysis yedata rekutanga velocity yakashandiswa kuona reagent substrate concentrations for assay ye enzyme iyi.Muedzo ukashandisa 4 mmol yegamma-glutamyl-3-carboxy-4-nitroanilide uye 100 mmol yeglycylglycine parita yakaburitsa zviitiko zvakafanana kune avo kuburikidza nekushandisa 4 mmol yegamma-glutamyl-4-nitroanilide uye 40 mmol glycylglycine parita.Aya maratidziro eiyo carboxy donor uye glycylglycine zvakare "inodhura zvakanyanya" uye haaratidze matambudziko emaitiro kana ashandiswa.
