PII chiratidzo processor mapuroteni akapararira zvakapararira muprokaryotes uye zvidyarwa kwaanodzora kuwanda kwevanabolic maitiro.Kubudirira kuwedzeredza kwematabolites kunoda kuzorodza iyo yakasimba cellular control circuits.Pano tinoratidza kuti imwe poindi shanduko muPII inoratidzira protein kubva ku cyanobacterium Synechocystis sp.PCC 6803 inokwana kuvhura nzira yearginine inokonzera kuunganidzwa kwebiopolymer cyanophycin (multi-L-arginyl-poly-L-aspartate).Ichi chigadzirwa ndechekufarira kwebiotechnological sesosi yemamino acids uye polyaspartic acid.Iri basa rinoratidza nzira itsva yenzira yeinjiniya nekugadzira tsika-yakagadzirirwa PII kusaina mapuroteni.Pano, iyo yakagadzirwa Synechocystis sp.PCC6803 strain ine PII-I86N mutation over-accumulated arginine kuburikidza ne constitutive activation yekiyi enzyme N-acetylglutamate kinase (NAGK) .Muinjiniya strain BW86, in vivo NAGK chiitiko chakawedzera zvakanyanya uye chakatungamira kune inodarika kagumi yepamusoro yearginine yemukati. kupfuura mhuka dzomusango.Nekuda kweizvozvo, rudzi rweBW86 rwakaunganidzwa kusvika pa57% cyanophycin pasero rakaomarara pasi pemamiriro akaedzwa, inova ndiyo goho repamusoro recyanophycin rataurwa kusvika parizvino.Strain BW86 yakagadzira cyanophycin mu molecular mass range ye 25 kusvika> 100 kDa;iyo mhuka yemusango yakagadzira iyo polymer muhuwandu hwe30 kusvika> 100 kDa. Kubereka kwepamusoro uye yakakwirira molecular molecular mass ye cyanophycin yakabudiswa nekuoma kweBW86 pamwe chete nezvinodiwa zvishoma zvinovaka muviri zve cyanobacteria zvinoita kuti zvive nzira inovimbisa yekugadzirwa kwebiotechnological ye cyanophycin.Ichi chidzidzo zvakare chinoratidza kugona kwemetabolism nzira engineering uchishandisa PII chiratidzo cheprotein, inoitika mune akawanda mabhakitiriya.
