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Protein phosphorylation inoronga mabasa akasiyana siyana uye inoita basa rakakosha mukukura kwezvirimwa.Kuzadzisa uye kuwedzera pane zvakamboferefetwa zveprotein phosphorylation mumichero yeArabidopsis takashandisa imwe nzira inosanganisa kubviswa kweprotein pasi pemamiriro asiri-denaturing neine immobilized metal-ion affinity chromatography (IMAC) kupfumisa kweintact phosphoproteins muRubisco-yakapera maextracts, ichiteverwa nekuzivikanwa. uchishandisa maviri-dimensional gel electrophoresis (2-DE) uye liquid chromatography-tandem mass spectrometry (LC-MS/MS).Mu-gel trypsin digestion uye kuongororwa kwemavara egel akasarudzwa akaonekwa 144 phosphorylated peptides uye zvakasara, izvo chete 18 phosphopeptides uye 8 phosphosites zvakawanikwa muPhosPhAt 4.0 uye P3DB Arabidopsis thaliana phosphorylation site databases.Inopfuura hafu ye82 yakaonekwa phosphoprotein yakabatanidzwa mu carbohydrate metabolism, photosynthesis / kufema kana oxidative stress response mechani sms.Kupfumiswa kwemaphosphoprotein isati yasvika 2-DE uye LC-MS/MS inoita seinosimudzira kuonekwa kwephosphorylated threonine uye tyrosine zvakasara zvichienzaniswa nenzira dzinoshandisa peptide-level hupfumi, zvichiratidza kuti nzira mbiri idzi dzinowirirana maererano nefosphorylation site coverage.Kuenzanisa mibairo yembeu diki neiyo yakambowanikwa kumashizha eArabidopsis akakura akacherekedza mapuroteni mashanu akasiyana phosphorylated mumatishu aya, zvichiratidza kugona kwehunyanzvi uhu hwekuferefeta kusimba kweprotein phosphorylation panguva yekukura kwechirimwa.