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Chemifluorescence: Molecular luminol ikemikari fluorescent molecule iyo inogona kushandurwa kuita yekunakidzwa mamiriro aminophthalic acid pamberi pehydrogen peroxide mamorekuru, ayo anoburitsa yakasimba fluorescence.Hydrogen peroxide ndiyo chigadzirwa chezvakawanda biooxidative reactions, saka zviri nyore kubatanidza izvi biooxidative reactions nephotodetection nekuunza luminol.Semuenzaniso, iyo glucose oxidase / catalase probe inogona kuona kuwanda kwehydrogen peroxide kana glucose mumuenzaniso, uye nguva yekupindura ndeye 0.5s chete (dynamic method).Kubatanidza chemiluminescent zvinhu neimmunological reactions, uye kuratidza kuunganidzwa kwezvakaonekwa zvikamu zvekudzivirira nekuita kwechiedza, kunonzi chemiluminescent immunoassay tekinoroji.Muna 1976, Shmeder akakurudzira chemiluminescence immunoassay, achishandisa luminol-H202 uye inotorwa kubva kuABEI seyekuona maitiro ekuratidzira system.Parizvino, luminol uye zvinobva mairi ndiyo inonyanya kushandiswa mamakisi muchemiluminescence immunoassay application.Pasi pemamiriro e-alkaline, akakonzerwa ne microperoxidase, nhamba huru yephotons inogona kusunungurwa.Luminol inogona kushandiswa kuWesternBlot yeHRP-labeled masoja ekudzivirira chirwere uye nucleic acid hybridization yekuona kwemaprobe akanyorwa neHRP.Inoshandiswawo mukuongorora kwemazuva ano tsotsi rekuona ropa.Mushonga unogona kushandiswa sechemiluminescent marker inofanira kusangana nemamiriro anotevera: quantum goho re luminescence yakakwirira;ayo physicochemical properties anofanana nehurongwa huri pasi pekudzidza;kupenya kwayo kunokonzerwa neiyo oxidation reaction yechinhu chine luminescent;mukati mehuwandu hwekugadzirisa hunoshandiswa Mukati husina chepfu kune zvipenyu.Mhando dzakawanda dzechemiluminescence reagents dzinowanzoshandiswa: acridine ester, inoshandiswa zvakanyanya chemiluminescence tracer, inonzi tricyclic organic compound, iri nyore kuoxidize, uye iyo oxidation reaction haidi catalyst, uye inobudisa mafotoni pa430nm;Luminol uye isoluminol uye zvinobva kwavari ndizvo zvakanyanya kukura chemiluminescent agents.Kare muna 1964, Ichiri kutaurwa nezve chemiluminescence, luminol, isoluminol uye zvakabva kwavari zvakashandiswa chemiluminescence.Immunoassays yakawana mhedzisiro iri nani.Kuonekwa kweLuminescence: iyo optimum fluorescence wavelength i400nm (kuonekwa kwechemiluminescence mu60mmK2S2O8, 100mmK2CO3, pH11.5 mhinduro)

Chemiluminescence: Senzira inonzwisisika yekuongorora, chemiluminescence inoshandiswa zvakanyanya kuona mahara radicals uye reaction metabolites inogadzirwa muma enzymes, masero uye zvipenyu zvipenyu.Chiedza chinoburitswa neavo oxidation zvigadzirwa uye metabolites inogona kushandiswa neakasiyana mafotometer.kuonekwa.Chemiluminescence inoshandiswa zvakanyanya mukuongorora uye kutsvagisa antioxidants, senge polyphenols, polysaccharides, flavonoids, uye anthraquinones, nekuda kwekunzwa kwayo, kukurumidza, kushanda kuri nyore uye mutengo wakaderera.Iyo inowanzo shandiswa chemiluminescence masisitimu ekugadzirisa superoxide anions anosanganisira xanthine oxidase-luminol, pyrogallol-luminol uye dimethyl sulfoxide-luminol.Iyo yekutanga inzymatic system, uye maviri ekupedzisira Iyo isiri-enzymatic system.Iyo chemiluminescence masisitimu ekugadzirisa kwehydroxyl radicals inonyanya kusanganisira copper sulfate-yeast (kana bone marrow masero) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen. peroxide 5 chemiluminescence systems, ferrous sulfate-luminol-hydrogen peroxide uye ferrous sulfate-luminol, zvose zvinosanganisira classic Fenton reaction kuti ibudise hydroxyl radicals, uye zvino kurwisa luminescent agent kuti ibudise chemiluminescence, inogona kushandiswa kuyera kubviswa kwezvinyorwa. basa reiyo reactive oxygen radicals.Nzira yekuedza: shandisa pyrogallol-luminol chemiluminescence system: luminol inogadzirwa mu 0.05mol / L yekugadzirisa mhinduro ine 0.05mol / L NaOH mhinduro, yakachengetwa munzvimbo ine rima, uye mvura yakasvibiswa kaviri isati yashandiswa Dilute ku1mmol / L solution, shandisa 1mmol / L HCl kugadzira 0.01mol / L mhinduro ye pyrogallol, ichengetedze mufiriji pa 4 ° C, uye inyungudze nemvura yakasvibiswa kaviri kusvika ku16 nguva 6.25 × 10-4mol / L mhinduro isati yashandiswa.0.05mol / L pH10.2Na2CO3-NaHCO3 buffer (ine 0.1mmol / LEDTA) yakagadzirwa isati yashandiswa, uye yakasanganiswa ne1mmol / L luminol pa2: 1 (chikamu chechikamu) kuti iite luminol uye carbonate buffer pamberi pekuedza musanganiswa.Munguva yekuyera, jekiseni 10.0 μL sampuli dzezvikamu zvakasiyana (0, 0.08, 0.4, 2 uye 10 mg/mL) musero re luminescent (ine sampuli buffer sekutonga), uye wobaya 6.25 × 10-4mol/L pyrogallol. 0.05 Pakupedzisira, 0.94 mL yemusanganiswa we luminol uye carbonic acid buffer yakawedzerwa kuti itange maitiro (30 ° C), simba re luminescence rakaverengwa pazvikamu zve 2 s, uye iyo yose yakasanganiswa luminescence intensity pa 300 s yakayerwa.Iyo yekumashure luminescence intensity yaive luminescence apo pyrogallol haina kuwedzerwa.value.Mukuwedzera, kune chemiluminescence, fluorescence spectroscopy uye NBS-dichlorofluorescein chemiluminescence system yekugadzirisa fluorescence chemiluminescence, nezvimwewo, mukana wavo mukuru ndeyekunzwa kwakanyanya.

Chemical Properties: Yellow crystalline poda.Inonyungudika zviri nyore mulye, inonyungudika mu dilute acid, inenge isinganyungudike mumvura, uye isinganyunguriki mudoro.Mishonga isina kwayakarerekera kana kuti ine acidic zvishoma inoratidza yakasimba yakajeka yeblue fluorescence kana yaiswa kune ultraviolet mwenje.Kunyungudika 329-332 ℃

Zvishandiso: Semakemikari ekuongorora reagent uye chiratidzo.Shandisa Chemiluminescence ongororo yekuongorora reagent (yakadai sekutemerwa kwesimbi cations kana ropa)

Zvishandiso: Yekuongorora chemiluminescence, senge: simbi cations, ropa uye glucocorticoids.

Zvishandiso: Luminescence test: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; mushure mekuwedzera H2O2) chemiluminescence reagent uye chiratidzo, chinowanzoshandiswa mukuongorora chemiluminescence, sesimbi cations, kudzivirirwa kweropa, nezvimwewo.