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1.Takadzidza inhibitory zvinokonzerwa neisorhamnetin pane mushroom tyrosinase ne inhibition kinetics uye computational simulation.Isorhamnetin reversibly inhibited tyrosinase nenzira yakasanganiswa-mhando paKi=0.235±0.013 mM.Zviyero zvemukati uye 1-anilinonaphthalene-8-sulfonate (ANS) -binding fluorescence yakaratidza kuti isorhamnetin haina kukonzera kuchinja kukuru muhutano hwepamusoro hwe tyrosinase.Kuti uwane nzwisiso mune yekusashanda maitiro, iyo kinetics yakaverengerwa kuburikidza nenguva-yepakati kuyerwa uye inoenderera substrate maitiro.Mhedzisiro yacho yakaratidza kuti kusashanda kwakakonzerwa neisorhamnetin yaive yekutanga-odha yekuita nebiphasic maitiro.Kuti tiwane imwe nzwisiso, takatevedzera docking pakati petyrosinase neisorhamnetin.Kutevedzera kwakabudirira (kusunga simba reDock6.3: -32.58 kcal / mol, ye AutoDock4.2: -5.66 kcal / mol, uye yeFred2.2: -48.86 kcal / mol), zvichiratidza kuti isorhamnetin inopindirana nemasara akawanda, akadai. seHIS244 uye MET280.Iri zano re predic ting tyrosinase kupindirana pamwe chete ne kinetics yakavakirwa pane flavanone komisheni inogona kuratidza kubatsira mukutarisa kune inogona zvisikwa tyrosinase inhibitors.

2. Acid unfolding pathway of conalbumin (CA), monomeric glycoprotein kubva kuhuku zai jena, yakaongororwa pachishandiswa kure- uye pedyo-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (Anilino-8-napthalene sulfonate) uye dynamic light scattering (DLS).Isu tinocherekedza pH-inotsamira shanduko mune yechipiri uye yepamusoro chimiro cheCA.Iyo ine yekuzvarwa-yakafanana α-helical yechipiri chimiro papH 4.0 asi kurasikirwa chimiro papH 3.0.Iyo CA yaivepo chete seyakanyungudutswa globule state uye yakanyungudutswa globule state mumhinduro papH 4.0 uye pH 3.0, zvichiteerana.Mhedzisiro yepH pane conformation uye thermostability yeCA inonongedza kune yayo yekudzivirira kupisa payakarerekera pH.DLS mhedzisiro inoratidza kuti MG nyika yaivepo seyakaomesesa fomu mune aqueous mhinduro ine hydrodynamic radii ye4.7 nm.Kudzimwa kwe tryptophan fluorescence ne acrylamide kwakawedzera kusimbisa kuunganidzwa kwenzvimbo yepakati, chikamu chakazarurwa, mukati-pakati penyika uye isina kuvhurika nyika.