4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Catalog Number | XD900018 |
| Product Name | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 |
| Storage Details | -15 kusvika -20 °C |
| Harmonised Tariff Code | 29400000 |
Chigadzirwa Specification
| Chitarisiko | Ichena kusvika kuOrange kusvika kuGreen powder kuita crystal |
| Assay | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is artificial substrate ye 4-nitrophenyl (pNP) glycopyranoside yekuona α-galactosidase basa.Kuwanda kwepNP yakasunungurwa kwakawedzera zvakanyanya apo 4-Nitrophenyl α-D-galactopyranoside yakashandiswa se substrate.
Kushandisa4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, the amountofliberatedpNPkubvaku4-Nitrophenylα-D-galactopyranosideinonyanya kukwirisa kupfuura kubva kuneimwepNP-glycopyranosidesatpH4.0apo pasina basarinopfuta-rinopfuta-rinopfuta. nyl (pNP) glycopyranosidesasartificial substrates inoratidza kutiyakasunungukagalactoseresidueskubvaku4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, kureva kutiHlevelwhereyieldthresholdisdecreasedastest.
Substrate ye α-D-galactosidase (α-D-Galactosidase).Inosungira kuE. coli lactose protein carrier.Pakati pe-α-D-galactose.
Nzira yekukurumidza yekuongorora lytic chiitiko cheantimicrobial agents kurwisa mbiriso uye fungi yakagadzirwa.Kuongorora kunobva pakuburitswa kweiyo intracellular enzyme, maltase (alpha-glucosidase).Chiitiko chakaburitswa chemaltase chakayerwa colorimetrically nekugadzirwa kwep-nitrophenol kubva p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Iyo lytic chiitiko cheakasiyana antimicrobial makomisheni akayerwa nembiriso maseru kana inomera spores yefilamentous fungi.Lytic anti-yeast chiitiko chinogona kuonekwa mukati me20 min incubation at 30 degrees C against Saccharomyces cerevisiae, Candida albicans, uye Cryptococcus neoformans.Lytic anti-fungal chiitiko chakaonekwa mushure memaawa maviri ekuvharirwa pa30 madhigirii C zvichipesana nezvinomera zvinomera zveAspergillus niger neBotrytis cinerea.Masero ese embiriso kana fungi haana hydrolyze yakakwana PNPG mukati me3 h pa30 madhigirii C kuti abudise shanduko yeruvara inooneka.Kuitwa kweLytic kwema enzymes (semuenzaniso, Lyticase), mishonga inorwisa mabhakitiriya (semuenzaniso, Amphotericin B), uye mhando yebhakitiriya inogadzira mishonga yakaonekwa pachishandiswa bvunzo.Iyo anti-yeast assay yakagadziridzwa kune 96-tsime microtiter fomati.Mayedzo ese ari maviri akapa kukurumidza, kunzwisisika, uye kudzokororwa kuona kwelytic anti-yeast uye anti-fungal chiitiko.
