4-Nitrophenyl phosphate disodium munyu 6-hydrate CAS:333338-18-4 chena kusvika kucheneruka yehupfu
| Catalog Number | XD90511 |
| Product Name | 4-Nitrophenyl phosphate disodium munyu 6-hydrate |
| CAS | 333338-18-4 |
| Molecular Formula | C6H16NNa2O12P |
| Molecular Weight | 371.142 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 29199000 |
Chigadzirwa Specification
| Chitarisiko | kuchena kusvika kucheneruka |
| Assay | 99% |
Pamusoro pe70% yegiredhi-giredhi gliomas inotakura heterozygous R132H mutation muiyo gene coding ye isocitrate dehydrogenase 1 (IDH1).Izvi zvinopa iyo enzyme ine riini kugona kushandura α-ketoglutarate kuenda ku2-hydroxyglutarate, inozotungamira kune tumorigenesis.Iyo huru sosi ye 2-hydroxyglutarate kugadzirwa ndeye glutamine, iyo, mugomarara, zvakare sosi ye tricarboxylic acid cycle (TCA) anaplerosis.Imwezve sosi yeanaplerosis ndeye pyruvate flux kuburikidza nepyruvate carboxylase (PC), inova nzira yakajairika mune yakajairika astrocytes.Chinangwa chechidzidzo ichi chaiva chekuona kana PC inoshanda sechikonzero cheTCA anaplerosis mu IDH1 mutant cells umo glutamine inoshandiswa 2-hydroxyglutarate production.Immortalized normal human astrocytes yakagadzirwa kuratidza heterozygous mutant IDH1 kana kuti mhuka dzomusango IDH1 dzakaongororwa.Kuyerera kwepyruvate kuburikidza nePC uye kuburikidza nepyruvate dehydrogenase (PDH) kwakatemwa nekushandisa magnetic resonance spectroscopy kuongorora kunyora kwe [2-¹³C]glucose-yakatorwa ¹³C-labe led glutamate uye glutamine.Mabasa ekuongorora, RT-PCR uye kumadokero blotting zvakashandiswa kuongorora kutaura uye kuita kwema enzymes akakodzera.Nhoroondo yeCancer Genome Atlas (TCGA) yakaongororwa kuti iongorore kutaura kwema enzymes muvanhu glioma samples.Kuenzaniswa nemasero emhando dzomusango, mutant IDH1 masero akawedzera zvakanyanya kuwedzera kwechikamu kuburikidza nePC.Izvi zvakabatana nekuwedzera kukuru kwePC chiitiko uye kutaura.Panguva imwecheteyo, basa rePDH rakaderera zvakanyanya, zvichida rakapindirana nekunyanya kuwedzera inhibitory PDH phosphorylation nePDH kinase 3. Zvinoenderana nekucherechedza mumasero, kuongororwa kweTCGA data yakaratidza kuwedzera kukuru kwePC kutaura mu mutant IDH-inoratidza vanhu glioma samples kana ichienzaniswa nemhuka- nyora IDH.Zvatinowana zvinoratidza kuti kuchinja muPC nePDH kunogona kuva chikamu chinokosha chekugadzirisa masero kune IDH1 mutation uye inogona kushanda sezvingatarisirwa kurapa.
