Iwo matsva eTrinder's reagents akanyanya kunyungudika mumvura aniline anobva ashandiswa zvakanyanya mukuongororwa kwekuongorora uye biochemical bvunzo.Pane zvakati wandei zvakanakira pamusoro pezvakajairwa chromogenic reagents mune colorimetric kutsunga kwehydrogen peroxide basa.Iwo matsva eTrinder's reagents akatsiga zvakakwana kuti ashandiswe mune zvese mhinduro uye yekuyedza pombi yekuona masisitimu.Pamberi pehydrogen peroxide uye peroxidase, riini reTrinder's reagent yakaratidzwa kuita ne 4- aminoantipyrine (4-AA) kana 3- methylbenzothiazole sulfonehydrazone (MBTH) panguva ye oxidative coupling reaction.Inogadzira violet yakagadzikana kana dhayi yebhuruu.Iyo molar absorbance yedhayi yakabatanidzwa neMBTH yaiva 1.5- 2 nguva yakakwirira kupfuura iyo yedhayi yakasanganiswa ne4-AA;zvisinei, mhinduro ye4-AA yakanga yakagadzikana kupfuura iyo MBTH mhinduro.Iyo substrate ine enzymatic oxidized neoxidase yayo kuti ibudise hydrogen peroxide.Iyo hydrogen peroxide concentration inoenderana neiyo substrate concentration.Naizvozvo, huwandu hwe substrate hunogona kutariswa nekukura kwemavara eiyo oxidative coupling reaction.Glucose, doro, acyl-CoA uye cholesterol inogona kushandiswa kuona iwo ma substrates akabatanidzwa kune inoveli Trinder's reagent uye 4-AA.Kune gumi matsva eTrinder's reagents aripo.Pakati pevatsva veTrinder's reagents, TOOS ndiyo inonyanya kushandiswa.Nekudaro, kune yakasarudzika substrate, kuyedza makirasi akasiyana einoveli Trinder's reagents inodiwa kuti ugadzire iyo yakakwana yekuona system.