Iyo itsva Trinder's reagent inonyungudika mumvura aniline derivative iyo inoshandiswa zvakanyanya mukuongorora kuongorora uye biochemical bvunzo.Iine mabhenefiti akati wandei pamusoro pezvakajairwa chromogenic reagents mune colorimetric kutsunga kwehydrogen peroxide basa.Iyo itsva Trinder's reagent yakagadzikana zvakakwana kune ese. Inogona kushandiswa mukugadzirisa kana muyedzo pombi yekuona system.Pamberi pehydrogen peroxide uye peroxidase, itsva Trinder'si reagent inogona kushandiswa pamwe chete ne 4-aminoantipyrine (4-AA) kana 3 methyl Panguva ye oxidative coupling reaction ye benzothiazole sulfonehydrazone (MBTH), yakagadzikana kwazvo yepepuru kana yebhuruu madhayi. zvinoumbwa.Iyo molar absorbance yedhayi yakasanganiswa neMBTH ndeye 1.5-2 nguva yakakwirira kupfuura iyo yedhayi yakasanganiswa ne4-AA;zvisinei, 4-AA Mhinduro yacho yakagadzikana kupfuura MBTH mhinduro.Iyo substrate ine enzymatic oxidized neoxidase yayo kugadzira hydrogen peroxide.Iko kusungirirwa kweiyi hydrogen peroxide kunoenderana nehuwandu hwe substrate.Naizvozvo, huwandu hweiyo substrate hunogona kutsanangurwa nekukura kwemavara eiyo oxidative coupling reaction.Glucose, doro, acyl-CoA, uye cholesterol inogona kushandiswa kuona iyo substrates yakasanganiswa neinoveli Trinder's reagents uye 4-AA.Kune gumi matsva eTrinder's reagents.Pakati pevatsva veTrinder's reagents, TOOS ndiyo inonyanya kushandiswa.Nekudaro, kune chaiyo substrate, kuyedza mhando dzakasiyana dzenovel Trinder's reagents zvinodikanwa kuti ugadzire iyo yakakwana yekuona system.
