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Kukuvadzwa kweoxidative kumabhesi eDNA kunowanzo konzera kuumbwa kweoxidized purines, kunyanya 7,8-dihydro-8-oxoguanine (8-oxoG) uye 7,8-dihydro-8-oxoadenine (8-oxoA), iyo yaimbova tsime- inozivikanwa mutagenic lesion.Sezvo 8-oxoG iri nyore kuzviisa pasi pekuwedzera oxidation kana ichienzaniswa neyakajairwa mabhesi, kuiswa kwechigadziko panguva yeDNA synthesis yakatarisana neiyo oxidized fomu ye8-oxoG yakaongororwa in vitro.A synthetic template ine imwechete 8-oxoG lesion yakatanga kurapwa neyakasiyana-elektroni oxidants kana pasi peiyo singlet mamiriro eokisijeni uye yakazoiswa pakuwedzera kweprimer yakagadziriswa neKlenow fragment exo- (Kf exo-), mhuru thymus DNA polymerase alpha (pol alpha ) kana DNA yemunhu polymerase beta (pol beta).Zvinoenderana nemishumo yapfuura, dAMP uye dCMP inoiswa yakasarudzika yakatarisana ne8-oxoG ine ese matatu maDNA polymerases.Sezvineiwo, oxidation ye8-oxoG yakawanikwa ichikonzera dAMP uye dGMP kuiswa kwakatarisana nechironda neKf exo- nekudziviswa kwechinguvana kwekuwedzera kweprimer o kuitika panzvimbo yenzvimbo yakagadziridzwa.Uyezve, chironda chinoumba chivharo panguva yeDNA synthesis nepol alpha uye pol beta.Maedzo ane 8-oxoA-modified template oligonucleotide inoratidza kuti zvese 8-oxoA uye oxidized fomu ye8-oxoA yakananga kuiswa kwedTMP neKf exo-.Misa spectrometric kuongororwa kwe8-oxoG-ine oligonucleotides isati yasvika uye mushure mekusanganiswa neIrCl62-inopindirana neoxidation yeinonyanya 8-oxoG saiti, zvichikonzera kuumbwa kweiyo guanidinohydantoin moiety sechigadzirwa chikuru.Hapana humbowo hwekuumbwa kwenzvimbo dzepasiki hwakawanikwa.Mhedzisiro iyi inoratidza kuti oxidized fomu ye8-oxoG, pamwe guanidinohydantoin, inogona kutungamirira zvisirizvo uye kusaisa dNTPs panguva yeDNA synthesis.Kana maitiro akadaro akaitika mu vivo, inomiririra poindi mutagenic lesion inotungamira kuG-->T uye G-->C shanduko.Nekudaro, iyo inoenderana oxidized fomu ye8-oxoA inonyanya kuratidza kwakaringana kuiswa kweT panguva yeDNA synthesis neKf exo-.