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Pathogenic molecular pathways mu cirrhotic chiropa zvirwere zvakadai sehepatitis C virus (HCV), autoimmune hepatitis (AIH) uye primary biliary cirrhosis (PBC) inoratidzwa zvisina kunaka.Majini akasiyana-siyana anoratidzwa anowanzo kukosha muchirwere chetachiona.Suppression subtractive hybridization (SSH) inzira ye genome-wide inopfumisa kune zvakasiyana-siyana zvinoratidzwa mRNA zvinyorwa.Isu takavavarira kuita tsvakiridzo nyowani yekusiyanisa gene kutaura mu cirrhosis tichishandisa SSH yakasanganiswa nequantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).Chiropa zvinyorwa muHCV cirrhosis, AIH cirrhosis, PBC, uye nondiseased chiropa tishu zvakaongororwa neSSH.Inokonzeresa inopindirana DNA (cDNA) clones yakadzokororwa kuti iratidzike mutsauko nedot-blot hybridization ndokuzoteedzerwa.Yakasarudzwa gene expression yakaverengerwa nechaiyo-nguva RT-PCR.Kutevera SSH, 694 clones yakadzokororwa kusiyanisa gene kutaura, iyo 145 yakatevedzana uye yakawanikwa ichitora kubva ku89 magene akasiyana.Makoni manomwe aive homologous chete ane yakaratidza kutevedzana tag (EST) sequences encoding majini asina basa rinozivikanwa.Up-regulated kutaura kwemajini mana kwakasimbiswa nechaiyo-nguva RT-PCR: transmembrane 4 superfamily nhengo 3 (tetraspanin CO-029) mune ese marudzi e cirrhosis, hedgehog inopindirana protein (HIP) muAIH cirrhosis uye chitinase 3-se-1. (HC gp-39 kana ykl-40) uye arginine-glutamic acid inodzokorora (RERE) muHCV cirrhosis.RERE gene polymorphisms uye splice akasiyana akaonekwa mune ese matissue akaongororwa.Tetraspanin CO-029 up-regulation yainyanya kuiswa kune bile ductular masero.Mukupedzisa, kucherechedzwa kutsva kwekusiyanisa gene kutaura mumunhu cirrhosis kwakaitwa pachishandiswa SSH sechinhu chekutanga chekuwana.Kunyanya, zvimwe zvidzidzo zveRERE gene uye zvigadzirwa zvayo muHCV yakabatana nechirwere chechiropa zvinotenderwa.