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Chinhu chisingatarisirwi chemamwe makirasi eanticoagulants anga ari osteoporosis inogona kunge iri, zvishoma muchikamu, ine chekuita ne deranged mesenchymal stem cell (MSC) basa.Chinangwa chechidzidzo chazvino chaive chekuenzanisa mashandiro efondaparinux (FDP), riwaya antithrombotic ine yechinyakare inoshandiswa zvakanyanya yakaderera molecular uremu heparin, tinzaparin (TZP) paMSC kuwanda uye kusiyanisa.MSCs dzakaparadzaniswa kubva kune trabecular bone ye14 trauma varwere ne collagenase-based digestion process uye yakawedzerwa mumamiriro akaenzana kusvikira ndima 3. Kuwedzera uye kusiyanisa kweMSCs kune chondrocytes uye osteoblasts yakaongororwa kana pasina kuwedzera kweFDP neTZP vachishandisa standard in vitro. kuyerwa uye kuwanda kwehuwandu hwehuwandu hwezvinodhaka.Flow cytometry yakashandiswa kuMSC phenotyping.Muzera rakadzidzwa boka (17-74 yemakore) iyo MSC frequency mucollagenase-yakabudiswa zvikamu zvaive 641 / 10 (6) masero (renji 110-2,158) uye maitiro avo ekukura aive ~4 mazuva / huwandu hwevanhu hukapetwa kaviri.Tsika dzine mwero MSC phenotype (CD73+, CD105+, CD146+, CD106+, uye CD166+).Kuwedzera kwesero kwakaongororwa nezvose zviri zviviri colony-forming unit-fibroblast (CFU-F) uye colorimetric tetrazolium munyu XTT assays.Mune ese maviri assay, kuwanda kweMSC kwakadziviswa nekuwedzera kweTZP, kunyanya pakakwirira.Kusiyana neizvi, FDP haina mhedzisiro pakuwedzera kweMSC.Osteogenic differentiation uye chondrogenic differentiation haina kukanganiswa nekuwedzera kweTZP kana FDP.Nepo kuwanda kweMSC, asi kusiri mutsauko, kukanganiswa neTZP, pakanga pasina humbowo hwemhedzisiro yeFDP mune ino in vitro modhi system inopokana nemashandisirwo ayo mugadziriro yemapfupa.