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Isu takagadzira chinyorwa chidiki chinopindira RNA (siRNA) yekutumira sisitimu tichishandisa ternary complex ine polyethyleneimine (PEI) uye γ-polyglutamic acid (γ-PGA), iyo yakaratidza kunyarara mhedzisiro uye isina cytotoxicity.Iwo mabinary complexes e siRNA ane PEI angangoita 73-102 nm muhukuru hukuru uye 45-52 mV mu ζ-inogoneka.Mhedzisiro yekunyarara yeSiRNA / PEI complexes yakawedzera nekuwedzera kwePEI, uye siRNA / PEI complexes ine chiyero chechaji yakakura kudarika 16 yakaratidza zvakakosha luciferase knockdown mu mouse colon carcinoma cell line inogara ichitaura luciferase (Colon26 / Luc cells).Zvisinei, yakasimba cytotoxicity uye ropa agglutination yakaonekwa mu siRNA / Lipofectamine yakaoma uye siRNA / PEI16 yakaoma.Recharging cationic complexes neanionic compound yakanzi inzira inovimbisa yekukunda huturu uhu.Saka takagadzirira ternary complexes ye siRNA ne PEI (charge ratio 16) nekuwedzera kwe γ-PGA kuderedza cytotoxicity uye kuendesa siRNA.Sezvaitarisirwa, iyo cytotoxicity ye ternary complexes yakaderera nekuwedzera kwe γ-PGA zvinyorwa, izvo zvakaderedza ζ-zvinogona zvezvakaoma.Mhedzisiro yakasimba yekunyarara yakafanana neye siRNA/Lipofectamine complex yakawanikwa mumaternary complexes anosanganisira γ-PGA ine anionic surface charge.Kubatanidzwa kwepamusoro kwe ternary complexes muColon26/Luc masero kwakasimbiswa nefluorescence microcopy.Mushure mekuwana kugogodza kwejini rakachinjika, kugona kweiyo yakaoma kuyananisira kudonha kweiyo endogenous yekuchengetedza gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), yakaongororwa muB16-F10 maseru.Iyo ternary complex (siRNA/PEI16/γ-PGA12 yakaoma) yakaratidza yakakosha GAPDH knockdown effect.Nekudaro, isu takagadzira inobatsira siRNA yekuendesa system.