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1.Metabolite profiling nzira dzakakosha midziyo yekuyera metabolite madziva mu biological systems.Kunyange zvazvo nzira dzakawanda dzekuongorora metabolite dzinoshuma kusimba kwehukama kana kutsamira pane zvishoma zvemukati zviyero zvinomiririra ese metabolites, chinodiwa chekupedzisira chetsanangudzo yehuwandu hwechidziva chemetabolite mumasero ehupenyu uye zvinwiwa ndezvekutsunga kwakadzama.Isu tinoshuma pano yakakwira-kuburikidza uye inonzwisisika gasi chromatography/tandem mass spectrometry (GC/MS/MS) yakanangwa metabolite profiling nzira inogonesa kukwana kwakazara kweese akaonekwa metabolites.Iyo nzira yakavakirwa pamethyl chloroformate derivatization uye quantification nespiking samples ine metabolite zviyero zvakapatsanurwa zvakabviswa ne deuterated derivatization reagents.Iyo yechinyakare erekitironi kukanganisa ionization inotsiviwa neyakanaka kemikari ionization sezvo iyo yekupedzisira kusvika pamwero wakakura ichichengetedza iyo molecular ion uye mamwe marefu mamorekuru huremu zvimedu.Izvi zvakaita kuti zvive nyore kusarudza yakasarudzika MS/MS shanduko pakati peakawanda coeluting metabolites.Parizvino, iyo novel GC/MS/MS nzira inosanganisira makumi matanhatu nenomwe akajairika ekutanga metabolites ayo mazhinji ari emapoka eamino uye nonamino organic acids.Isu tinoratidza kushanda kweiyo nzira pane weti uye serum samples.Iyo nzira ndeye kunatsiridza kwakakosha kwezvino nzira yehuwandu GC/MS metabolite profiling yeamino acids uye nonamino organic acids.

2.24S-Hydroxycholesterol (24OHC) uye 27-hydroxycholesterol (27OHC) maoxysterol maviri akafanana ane mabviro akasiyana-aimbova anenge akaumbwa muuropi chete uye ekupedzisira akaumbwa zvishoma muuropi kupfuura mune dzimwe nhengo dzakawanda.HYPOTHESIS INOZOYEDZWA: Neuronal kukuvadzwa uye/kana demyelination kunokonzera kuwedzera kuyerera kwe24OHC kubva muuropi kuenda mucerebrospinal fluid (CSF), nepo kukanganiswa kweropa-brain barriers kunokonzera kuwedzera kuyerera kwe27OHC kubva mukutenderera muCSF.Isotope dilution-mass. spectrometry yakashandiswa kuongorora maoxysterols maviri muCSF uye plasma kubva kune vanopfuura 250 varwere vane zvirwere zvakasiyana-siyana zvetsinga uye geriatric.Mazinga eCSF emaoxysterol maviri akanyanya kukanganiswa nezvirwere zvakasiyana-siyana kupfuura mazinga eplasma.Varwere vane zvirwere zvinogadzirisa zvirwere vakawedzera mazinga e24OHC muCSF ine chiyero chepamusoro che 24OHC / 27OHC.Varwere vane meningitis kazhinji vaive nemazinga akakwira ese ari maviri steroid ane yakaderera 24OHC/27OHC reshiyo.Varwere vane chirwere cheAlzheimer vakawedzera zvishoma mazinga e24OHC muCSF nekuwedzera kushoma mu27OHC.Varwere vane multiple sclerosis vaiva netsika yekuva nemazinga akakwirira e24OHC panguva dzekushanda nepamusoro 24OHC / 27OHC ratio.