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Ongororo yezviitiko zveproteome-wide phosphorylation ichiri dambudziko guru rekuongorora nekuda kwekuoma kwakakura kweprotein phosphorylation network.Mubasa iri, tinoongorora kuwirirana kweLys-N, Lys-C, uye trypsin maererano nekugona kwavo kubatsira mukuongororwa kwepasi rose kwephosphoproteome.Shanduro yakanatswa yepasi-pH yakasimba cation exchange yakashandiswa kunyatsoparadzanisa N-terminally acetylated, phosphorylated, uye nonmodified peptides.Zvose zve 5036 zvisingasviki phosphopeptides zvinogona kuonekwa nehuwandu hwekuwanikwa kwenhema kwe <1% kubva ku 1 mg yeprotein uchishandisa kusanganiswa kwema enzymes matatu.Nhoroondo yedu yakaratidza kuti kupindirana pakati pephosphopeptide data seti yakagadzirwa neproteases yakasiyana yakanga iri pamucheto, asi kupindirana pakati pemaviri akafanana akagadzirwa tryptic data seti yakawanikwa iine kanenge 4 nguva yakakwirira.Nenzira iyi, kushandiswa kwakafanana kweLys-N uye trypsin kwakagonesa kuwedzera kwe72% muhuwandu hweanoonekwa phosphopeptides sekuenzanisa d netrypsin chete, nepo trypsin replicate kuyedza kwakangotungamira kune 25% kuwedzera.Nekudaro, kana tichingotarisa chete pane trypsin neLys-N data, takaona 4671 nonredundant phosphopeptides.Kumwe kuongorora kwenzvimbo dzakaonekwa kwakaratidza kuti Lys-N uye trypsin data seti dzakafumiswa mune zvakasiyana zvakanyanya phosphorylation motifs, zvichiratidza kuti nzira dzemultiprotease dzakakosha zvakanyanya mukuongorora phosphoproteome.