L-Proline Cas: 147-85-3 99% White powder
| Catalog Number | XD90293 |
| Product Name | L-Proline |
| CAS | 147-85-3 |
| Molecular Formula | C5H9NO2 |
| Molecular Weight | 115.13046 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 29339980 |
Chigadzirwa Specification
| Assay | 99% min |
| Chitarisiko | Upfu chena |
| Kutenderera chaiko | -84.5 kusvika -86 |
| Heavy metals | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Kurasika paKuomesa | <0.3% |
| Residue paIgnition | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| State of Solution | >98% |
Kunzwisisa mametabolism eiyo microbial host kwakakosha mukusimudzira uye optimization ye-sero-yakavakirwa biocatalytic maitiro, sezvo inoraira kugadzirwa kwakanaka.Izvi ndezvechokwadi kunyanya kune redox biocatalysis uko masero anoshanda metabolic anoshandiswa nekuda kweiyo cofactor/cosubstrate regenerative kugona endogenous mune inotambira.Recombinant Escherichia coli yakashandiswa pakuwandisa proline-4-hydroxylase (P4H), dioxygenase catalyzing the hydroxylation of free L-proline into trans-4-hydroxy-L-proline with a-ketoglutarate (a-KG) secosubstrate.Mune iyi yese-sero biocatalyst, yepakati kabhoni metabolism inopa inodiwa cosubstrate a-KG, kubatanidza P4H biocatalytic performance yakananga kucarbon metabolism uye metabolic chiitiko.Nekushandisa zvese zvekuedza uye computational biology maturusi, akadai se metabolic engineering uye (13) C-metabolic flux analysis ((13) C-MFA), takaferefeta uye tikatsanangura zvakanyanya physiological, metabolic, uye bioenergetic mhinduro yeiyo yese-sero biocatalyst. kune yakanangwa bioconversion uye yakaona zvingangove zvipingamupinyi zvemetabolism yeimwe nzira ine musoro engineering.A proline degradation-inoshaya E. coli strain yakagadzirwa nekudzima putA gene encoding proline dehydrogenase.Yese-sero biotransformations neiyi mutant strain yakatungamira kwete chete kuwanda kweproline hydroxylation asiwo nekupeta kweiyo chaiyo trans-4-L-hydroxyproline (hyp) yekugadzira mwero, zvichienzaniswa nemhando yemusango.Ongororo yekabhoni flux kuburikidza nepakati metabolism yeiyo mutant strain yakaratidza kuti yakawedzera a-KG kudiwa kweP4H chiitiko haina kuwedzera a-KG inogadzira flux, ichiratidza yakanyatso kudzorwa TCA kutenderera kushanda pasi pemamiriro akadzidzwa.Mumhando yemusango, P4H synthesis uye catalysis yakakonzera kudzikiswa kwegoho rebiomass.Sezvineiwo, iyo ΔputA strain yakawedzera kutsiva iyo yakabatana ATP uye NADH kurasikirwa nekudzikisa kuchengetedza kwesimba zvinodiwa payakafanana yakaderera glucose kutora mazinga, pane kuwedzera TCA chiitiko.The putA knockout in recombinant E. coli BL21 (DE3) (pLysS) yakawanikwa kuve kuvimbisa kugadzirwa kweP4H catalysis kwete chete maererano neiyo biotransformation goho, asiwo maererano nemitengo ye biotransformation uye proline uptake uye goho re hyp pane simba sosi.Mhedzisiro yacho inoratidza kuti, pane putA knockout, kubatana kweTCA-cycle kuproline hydroxylation kuburikidza necosubstrate a-KG inova chinhu chakakosha chinomanikidza uye chinangwa chekuwedzera kuvandudza kushanda kwe-KG-inotsamira biotransformations.
