Products

Munzira yekinetic angiotensin-converting enzyme (ACE), inoshanda uye yakakwana buffer i80 mmol/L borate buffer papH 8.2 (37 degrees C).Chikamu chelag chinoonekwa mukuita, uye 5-min incubation ye substrate uye plasma inokurudzirwa pamberi peyero kinetic.Iyo substrate, N--[3- (2-furyl) acryloyl] -L-phenylalanylglycylglycine (FAPGG), fungidziro inokwidziridzwa pa1.0 mmol/L uye kuyerwa kwewavelength iri pa345 nm kuti ive nechokwadi chekuyera mutsara.Maitiro akatsanangurwa anoshandisa 1: 9 plasma-to-reagent volume ratio.Mutsara wemutsetse weassay unosvika kusvika pa170 U/L, inomiririra 25% substrate hydrolysis.Iyo FAPGG absorptivity inotariswa nekuyera mutsauko mukunyudza pakati pe1.0 mmol/L FAPGG uye zvigadziriso zvechigadzirwa.Kuvimbika kwewavelength kunotariswa nekucherechedza iyo inotarisirwa kunyura kukosha kweiyo FAPGG mhinduro, uye 1.0-nm kutsauka kubva ku345 nm inoshandura kunyura ne15.5%.Iyo chaiyo yeACE assays pane angangoita 60 uye zana U/L ndeye 3.5% uye 2.4% mukati mebatch uye 2.9% uye 2.6% pakati pebatch, zvichiteerana.Nguva yereferensi (2.5th kusvika 97.5th percentiles) ndeye 41-139 U/L, uye hapana mutsauko pakati pehunhu hwevarume nevakadzi.