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Kune magamba mazhinji asina kuimbwa akatitenderedza, anoita seakangojairika, asi kutaura zvazviri anogovera zvakawanda kwatiri chinyararire.Proteinase K ndiye "gamba risingazivikanwe" muindasitiri yekuongorora mamorekuru, kunyangwe ichienzaniswa ne "hombe uye ine simba" muindasitiri, proteinase K yakaderera-kiyi zvekuti isu takaregeredza kukosha kwayo.Nekubuda kwedenda idzva rekorona, kudiwa kweproteinase K kwakawedzera, uye kuwanikwa kumba nekune dzimwe nyika kuri kumashure kwekushandiswa, uye munhu wese akaerekana aona kuti proteinase K yakakosha.
Chii chinonzi proteinase K
Proteinase K is serine protease ine proteolytic enzyme basa uye inogona kuchengetedza basa munzvimbo dzakasiyana-siyana (pH (4-12.5), high-salt buffer, kupisa kwepamusoro kwe70 ° C, nezvimwewo).Mukuwedzera, basa reproteinase K harina kudziviswa neSDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, nezvimwewo, uye imwe yakawanda ye detergent inogonawo kuwedzera basa reprotease K. Mukurapa kwemishonga (virusi uye microbial disinfection). ), chikafu (nyama nyoro), dehwe (kunyorovesa bvudzi), winemaking (doro kujekesa), amino acid kugadzirira (yakaora minhenga), nucleic acid extraction, in situ hybridization, nezvimwewo, proteinase K Pane zvikumbiro.Iyo inonyanya kushandiswa application ndeye nucleic acid yekubvisa.
Proteinase K inogona enzymolyze marudzi ese emapuroteni mumuenzaniso, kusanganisira ayo histones akasungwa zvakasimba kune nucleic acids, kuitira kuti nucleic acids isunungurwe kubva mumuenzaniso uye ibudiswe muchidimbu, kufambisa danho rinotevera rekubvisa nekucheneswa.Mukuonekwa kwehutachiona nucleic acid, proteinase K chimwe chezvinhu zvakakosha mumhinduro yesampling yehutachiona.Proteinase K inogona kuputika uye kusaita puroteni yejasi yehutachiona, iyo yakachengeteka panguva yekufambisa uye yekuona danho;uyezve, proteinase K inogona zvakare kushatisa RNase inodzivirira kuderedzwa kwehutachiona RNA uye inofambisa kuona nucleic acid.
Mukurumbira wehusiku hweproteinase K
Ingave mumunda wekutsvagisa kwesainzi kana mundima yeIVD, kutorwa kweasiti nucleic ndiyo yakanyanya kuyedza, saka proteinase K yagara iri hupenyu hwakakosha.Zvisinei, munguva yakapfuura, proteinase K yakanga isinganyanyi kuzivikanwa pane basa rayo.Chikamu chikuru cheizvi ndechekuti hukama hwekugovera uye hwekudiwa hweproteinase K hwakanga hwakagadzikana.Vashoma vanhu vangafunga kuti kupihwa kweproteinase K kungave dambudziko.
Nekubuda kwedenda idzva rekorona, kudiwa kweiyo nucleic acid kuyedzwa kwawedzera.Kubva pakupera kwaChikumi 2020, China yapedza bvunzo dzinosvika mamirioni makumi mapfumbamwe ekorona, uye nhamba iyi iri kutonyanya kutyisa pasi rose.Mune nucleic acid yekubvisa zviedzo, iyo yekushanda yeproteinase K inenge 50-200 μg/mL.Kazhinji, zvinotora anenge 100 μg yeproteinase K kubvisa sampuli ye nucleic acid.Mukushandiswa kwechokwadi, kuitira kuti kuwedzere kushanda kwe nucleic acid kubudiswa, kazhinji Proteinase K ichashandiswa muhuwandu hwekuwedzera.Nucleic acid yekuonekwa kweiyo coronavirus itsva yakaunza huwandu hukuru hweproteinase K kudiwa.Iyo yepakutanga yekupihwa uye yekuda chiyero cheproteinase K yakakurumidza kutyorwa, uye proteinase K yakave yakakosha kudzivirira denda usiku humwe.
Matambudziko mukugadzira proteinase K
Kunyangwe nekuvandudzwa kwedenda, kukosha kwakakosha kweproteinase K kwakakosheswa nevanhu, zvinonyadzisa kuti nekuda kwekunyanya kuderera-kiyi yeprotease K, mashoma makambani epamba akabatanidzwa mukugadzirwa kweproteinase K. Apo vanhu vanoda kumisikidza proteinase K kugadzirwa Panguva yekugadzira, zvakaonekwa kuti proteinase K ipuroteni yakakosha zvakanyanya.Izvo zvakanyanya kunetsa kuwedzera simba rekugadzira reproteinase K munguva pfupi.
Kugadzirwa kukuru kweproteinase K kunotarisana nematambudziko anotevera
1. Kutaura kwakaderera
Proteinase K inogona kusanyanya kushatisa mapuroteni mazhinji uye kukonzera huturu hwakakomba kune yekutaura host cell.Naizvozvo, chiyero chekutaura cheproteinase K kazhinji chakaderera.Kuongororwa kwemaitiro ekutaura uye madhiri anoratidza zvakanyanya proteinase K kazhinji inoda kutenderera kwakareba.
2. Zvisaririra zvepigment uye nucleic acids
Kuvirisa kwakakura kunounza huwandu hukuru hwe pigment uye inotambira nucleic acid zvisaririra.Zvakaoma kubvisa tsvina iyi nemaitiro akareruka ekuchenesa, uye kucheneswa kwakaoma kunowedzera mutengo uye kunoderedza chiyero chekudzorera.
3. Kusagadzikana
Proteinase K haina kugadzikana zvakakwana, inogona kuita enzymolyze pachayo, uye zvakaoma kuichengeta yakadzikama pa 37 ° C kwenguva yakareba pasina mumiririri anodzivirira.
4. Nyore kunaya
Paunenge uchigadzira iyo yakaomeswa-yakaomeswa poda yeproteinase K, kuitira kuti uve nechokwadi chekuti iyo yakasimba yeproteinase K muhupfu yakaomeswa yakakura, zvinodikanwa kuwedzera inodzivirira-yakaomeswa inodzivirira pane yakanyanya kusungwa, asi kana concentration yeproteinase K inosvika 20mg/mL uye pamusoro, zviri nyore Iko kuunganidza kunoumba mvura, iyo inounza matambudziko makuru pakuomesa-kuoma kweproteinase K ine yakakwirira yakasimba.
5. Mari yakakura
Proteinase K ine basa rakasimba reprotease uye inogona kuita hydrolyze mamwe maprotease murabhoritari.Naizvozvo, proteinase K inoda nyanzvi dzekugadzira nzvimbo, zvishandiso, uye vashandi vekutsvaga nekusimudzira nekugadzira.
XD BIOCHEM's proteinase K mhinduro
XD BIOCHEM has a mature protein expression and purification platform, and has rich experience in the expression and purification of recombinant proteins and optimization of production processes. Through the rapid formation of a research and development team, the large-scale production process of proteinase K has been overcome. The monthly output of freeze-dried powder is more than 30 KG. The product has stable performance, high enzyme specific activity, and no host cytochrome and nucleic acid residues. Welcome to contact XD BIOCHEM Obtain a trial package (E-mail: sales@xdbiochem.com Tel: +86 513 81163739).
XD BIOCHEM's tekinoroji mhinduro dzinosanganisira
Kushandisa ma-multi-copy plasmid kubatanidzwa, maitiro e-high-expression ane maitiro ekutaura kwe8g / L anosarudzwa, izvo zvinokunda dambudziko rekuderera kwekutaura kweproteinase K;
Kuburikidza nekugadzwa kwematanho akawanda ekuchenesa nzira, iyo host cytochrome uye nucleic acid zvakasara zveproteinase K zvakabudirira kubviswa pasi pehuwandu hunokosha;
Kuburikidza nepamusoro-soro yekuongorora kwemaitiro ekudzivirira ekudzivirira, buffer inogona kuchengetedza zvakagadzikana proteinase K pa 37 ° C yakasarudzwa;
Kuongorora mabhafa kunokunda dambudziko rekuti proteinase K iri nyore kuunganidza uye kunaya pakakwirira, uye inoisa hwaro hweproteinase K's high solid content kuomesa-kuomesa.

XD BIOCHEM proteinase K sampuli

XD BIOCHEM proteinase K kugadzikana bvunzo: hapazove nekuchinja kwakakosha muchiitiko mushure me80 d pakupisa kwekamuri.

XD BIOCHEM proteinase K kugadzikana bvunzo: hapazove nekuchinja kwakakosha muchiitiko mushure me80 d pakupisa kwekamuri.

Kuenzanisa kweiyo nucleic acid yekubvisa mhedzisiro yeXD BIOCHEM proteinase K uye zvigadzirwa zvinokwikwidza.Mukuita kwekubvisa nucleic acid, XD BIOCHEM uye yemakwikwi proteinase K inoshandiswa zvakateerana.Iko kugona kwekubvisa kweXD BIOCHEM proteinase K kwakakwira uye kukosha kweCt kweiyo gene gene yakaderera.