ONPG CAS:369-07-3 98.0% Min White Kuenda Kure -White Powder
| Catalog Number | XD90006 |
| CAS | 369-07-3 |
| Product Name | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 |
| Storage Details | 2 kusvika ku8 °C |
| Harmonised Tariff Code | 29400000 |
Chigadzirwa Specification
| Kuchena (HPLC) | Min.98.0% |
| Chitarisiko | Muchena kusvika kure-chena hupfu |
| Solution(1% mumvura) | Yakajeka, isina ruvara kusvika kune yero zvishoma |
| Mvura Yemukati(Karl Fisher) | Max.0.5% |
| Kutenderera kwemaziso chaiwo [α]D20(c=1, H2O) | - 65.0 ° C kusvika -73.0 ° C |
Hurukuro yeONPG bvunzo (β-galactosidase bvunzo)
Mibvunzo yagara ichimutswa nguva pfupi yadarika: 1. Sei bvunzo yeONPG ichigona kushandiswa kusiyanisa kunonoka kuviriswa kwelactose?2. Sei chiyero chenyika chichiti zvakakosha kushandisa 3% sodium chloride trisaccharide iron (kana trisaccharide iron) pabvunzo yeONPG?3. KuVibrio parahaemolyticus, pakuita bvunzo yeOPNG, sei toluene ichifanira kuwedzerwa kudonha zvichienderana nechiyero?Basa racho nderei?
Kambani yedu yakaongorora ruzivo rwakawanda, yakapfupisa, uye yakagovana newe pazasi:
Musimboti: Zita rechiChinese reONPG ndere o-nitrobenzene-β-D-galactopyranoside.ONPG inogona kuiswa hydrolyzed kuita galactose uye yero o-nitrophenol (ONP) ne β-galactosidase, saka chiitiko che β-galactosidase chinogona kuonekwa nekushanduka kweruvara rwetsika yepakati.
Lactose ishuga iyo tupukanana zhinji inoda kuona.Iyo metabolism inoda ma enzymes maviri, imwe sero permease, lactose inopinda mumasero pasi pechiito chepermease;imwe yacho β-galactosidase, iyo inogadzika lactose kuita galactose.Lactose uye glucose.β-Galactosidase inogonawo kuita zvakananga paONPG kuita hydrolyze kuita galactose uye yero o-nitrophenol (ONP).Inogona kuitwa mu24h, kunyangwe nelactose yakanonoka kuvirisa.Nokudaro, inotsanangura migumisiro yekucherechedza yekutora tsika 1 kubva kune agar slant uye kuipinza mudenderedzwa rakazara pa 36 ° C kwe 1-3h uye 24h.Kana β-galactosidase inogadzirwa, inoshanduka yero mu 1-3h, kana pasina enzyme yakadaro, haizochinji ruvara mu24h.
Zvinoenderana nema enzymes maviri ari pamusoro, ma microorganisms anogona kukamurwa muzvikamu zvinotevera:
1 lactose-fermenting (18-24 maawa) mabhakitiriya ane permease uye β-galactosidase P + G +;
2 Inononoka lactose fermenters (inotora nguva yakareba kupfuura maawa makumi maviri nemana) ichishaya permease asi ine galactosidase: P- G+.
3 Non-lactose fermenters inoshaya zvose permease uye galactosidase: P- G-.
Iyo ONPG bvunzo inogona kushandiswa kusiyanisa lactose-lag-fermenting mabhakitiriya (P-G+) kubva kune asiri kuvirisa lactose mabhakitiriya (PG-), akadai se:
1 Kusiyanisa kunonoka lactose fermenters (P- G+) kubva kune isiri-lactose fermenters (P- G-).
(a) Citrobacter (+) uye Salmonella arizonae (+) kubva kuSalmonella (-).
(b) Escherichia coli (+) kubva kuShigella sonnei (-).
Nei iyo ONPG assay yakaitwa pachishandiswa tsika yehusiku pa3% sodium chloride ferric trisaccharide (iron trisaccharide)?Kambani yedu yakabvunza ruzivo rwakawanda, asi hapana chirevo chakajeka.Chete padandemutande reFDA, pakanyorwa kuti "Inoculate tsika dzinofanirwa kuongororwa katatu shuga iron agar slants uye incubate kwemaawa gumi nemasere pa37 ° C (kana imwe tembiricha yakakodzera, kana zvichidikanwa) . Nutrient (kana imwe) agar slants ine 1.0 % lactose inogonawo kushandiswa."zvinoreva: mabhakitiriya ekuedzwa akaiswa pane trisaccharide iron medium uye akagadzirwa pa 37 ° C kwe18h.Nutrient agar slants (kana imwe) yepakati ine 1% lactose inogamuchirwa zvakare.Nokudaro, zvinofungidzirwa kuti trisaccharide iron medium ine lactose.Mushure mekukura kwehusiku, mabhakitiriya akagadzira yakanaka inoshanda β-galactosidase.Uchishandisa mabhakitiriya akadai, ONPG inogona kuora ne β-galactosidase nekukurumidza.Chiitiko chekuedza chinokurumidza uye chinoratidzwa zviri nani.Pamusoro pezvo, kuwedzera kwedonhwe re toluene uye kugeza kwemvura kwemaminetsi mashanu zvese kuitira kuburitsa zvizere β-galactosidase.
