PIPES Cas: 5625-37-6 White crystalline poda 99% ABTS DIAMMONIUM MUNYU ULTRA PURE GRADE
| Catalog Number | XD90117 |
| Product Name | PIPES (Piperazine-1,4-bis(2-ethanesulfonic acid)) |
| CAS | 5625-37-6 |
| Molecular Formula | C8H18N2O6S2 |
| Molecular Weight | 302.37 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 2933599 |
Chigadzirwa Specification
| Heavy metals | <5ppm |
| Kurasika paKuomesa | <1.0% |
| Solubility | Yakajeka, isina ruvara mhinduro (5% 1N NaOH) |
| Assay | 99 - 101% |
| Chitarisiko | White crystalline powder |
PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] inowanzoshandiswa sechinhu chinovhara mubiochemistry.Iyo ethanesulfonic acid buffer yakagadzirwa naGood et al.muma1960.PIPES ine pKa padhuze nefiziological pH inoita kuti ibatsire musero tsika basa.Izvo zvakanyorwa kuti zvideredze kurasikirwa kwelipid paunenge uchibhafa glutaraldehyde histology mumiti uye mhuka tishu.A buffering agent ine pKa pedyo nephysiological pH.
Antimicrobial peptide magainin 2 inoumba pores mu lipid membranes uye induces membrane permeation yemukati meserura.Kunyangwe kupindira uku kuri iko kukonzeresa kwekuita kwayo kwebhakitiriya, maitiro ekugadzira pore anoramba asinganzwisisike.Saka takaongorora zvakadzama kudyidzana kwegainin 2 ine lipid membranes tichishandisa imwechete hofori unilamellar vesicles (GUVs).Kusungirirwa kwemainin 2 kune lipid membrane yeGUVs kwakawedzera kuchinja kwechikamu munzvimbo ye membrane, δ, iyo yakanga yakaenzana nepamusoro pehuwandu hwemagiinin 2, X. Izvi zvinoratidza kuti chiyero chenguva dzose chemugain 2-induced two. -kushanduka kwenyika kubva kune intact state kuenda kune pore state yakawedzera zvakanyanya nekuwedzera kwe δ.Iko kunetsana kweiyo lipid membrane inotevera kushuvira kweGUV zvakare yakagadziriswa magainin 2-induced pore kugadzirwa.Kuratidza nzvimbo yemagazini 2, kudyidzana kwecarboxyfluorescein (CF)-yakanyorwa magainin 2 (CF-magainin 2) ine maGUVs ega ane mvura inonyungudika nefluorescent probe, AF647, yakaongororwa pachishandiswa confocal microscopy.Mukushaikwa kwekukakavadzana nekuda kwechishuwo, mushure mekubatana kwemagazini 2 iyo fluorescence intensity yeGUV rim nekuda kweCF-magainin 2 yakawedzera nekukurumidza kusvika kune yakadzikama kukosha, iyo yakaramba yakasimba kwenguva yakareba, uye pa4-32 s pamberi. kutanga kwekudonha kweAF647 iyo rim intensity yakatanga kuwedzera nekukurumidza kune imwe yakadzika kukosha.Kusiyana neizvi, muhupo hwekukakavadzana, hapana kuwedzera kwekusimba kwerimu nguva isati yatanga kubuda yakaonekwa.Mhedzisiro iyi inoratidza kuti magain 2 haigone kutama kubva kunze kuenda kune yemukati monolayer kusvika nguva pfupi isati yagadzirwa.Kubva pamigumisiro iyi, tinogumisa kuti magainin 2-induced pore inzvimbo yakatambanudzwa-yakagadziriswa uye kutambanudza kwemukati monolayer ndiyo inonyanya kufambisa simba rekugadzira pore.
