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Kusiyanisa kwe orbital fibroblasts kuita adipocytes yakakura uye kuunganidza kwakatevera kweadipose tissue kwakaratidzwa mukufambira mberi kweGraves 'orbitopathy (GO).Autophagy inobatanidzwa mune adipogenesis, asi zvishoma zvinozivikanwa nezve basa re autophagy mukutanga uye kufambira mberi kweGO.Chinangwa chechidzidzo ichi ndechekuongorora basa re autophagy mupathogenesis yeGO.Orbital adipose / connective tissue explants kubva kuvarwere vane GO uye kubva kune zvidzidzo zvemazuva ose, pamwe chete neyoga orbital fibroblasts, yakaongororwa.Adipogenesis yakaitwa ichishandisa kusiyanisa svikiro ine kana isina hydrogen peroxide, uye autophagy yakashandiswa uchishandisa bafilomycin A1 uye Atg5-yakanangwa pfupi hairpin RNA (shRNA).Autophagosomes yakaonekwa ne electron microscopy.Tsanangudzo yeautophagy-ane chekuita nemajini uye adipogenesis-ane chekuita nezvinyorwa zvinyorwa zvakaongororwa nenguva chaiyo reverse transcription-polymerase chain reaction uye/kana Western blot analysis.Lipid droplet accumulation yakaongororwa neOil Red O staining.Autophagic vacuoles aive akawanda muGO masero pane asiri maGO masero (p <0.05).Kuratidzwa kwemajini ane chekuita neautophagy kwaive kwakakwira zvakanyanya muGO tishu uye maseru pane kune avo asiri-GO vamwe vavo, zvichiteerana.Interleukin-1β yakawedzera LC3-II, p62, uye Atg7 protein muGO masero.Autophagosome kuunganidza yakaratidzwa pazuva 4 yeadipogenesis uye yakaderera nezuva 10, pamwe chete ne lipid droplet formation.Kuratidzwa kweLC3 uye p62 mapuroteni akawedzera mukati maawa 48 ekusiyanisa uye akaderera zvishoma nezvishoma kubva pazuva 4 kusvika 10. Bafilomycin A1 kurapwa uye Atg5 knockdown ne shRNA inhibited lipid droplet accumulation uye kudzvinyirirwa kutaura kweadipogenic markers.Autophagy yakawedzerwa muGO tissue uye masero akaenzaniswa ne asiri-GO tishu uye masero, zvichiratidza kuti autophagy inoita basa muGO pathogenesis.Autophagy manipulation inogona kunge iri yekurapa chinangwa cheGO.