Products

A very sensitive microRNA (miRNA) biosensor inoshandisa ruthenium oxide nanoparticle (RuO(2) NP) -yakatangwa polymerization ye3,3'-dimethoxybenzidine (DB) uye miRNA-templated deposition ye insulating poly(3,3'-dimethoxybenzidine) (PDB) firimu rinotsanangurwa mubasa iri.Iyo biosensor yakagadzirwa nemusanganiswa monolayer ye oligonucleotide capture probes (CPs) uye 4-mercaptoaniline pane electrode yegoridhe.Zvichitevera kusanganiswa neRuO(2) NP-tagged target miRNA, musanganiswa weDB/H(2)O(2) mupH 5.0 0.10 M acetate buffer wakaiswa kune biosensor.Iyo RuO (2) NPs inoshanda sepolymerization initiator/catalyst yepolymerization yeDB.Uye iyo yakasanganiswa anionic miRNA tambo uye yemahara maCP anoshanda sematemplate, achitungamira kuiswa kwePDB.Huwandu hweiyo yakaiswa PDB uye simba rayo rekudzivirira rakabatana zvakananga kune iyo yakanangwa miRNA mumhinduro.Electrochemical impedance spectroscopic tests yakaratidza kuti linear charge-transfer resistance-concentration relationship kubva pa6 .0 fM kusvika 2.0 pM yakawanikwa mushure me 60 min of incubation muDB/H (2) O (2) musanganiswa.Pakanga pasina muchinjika-kusanganiswa pakati pe-pre-miRNA uye miRNA yakakura uye kushoma-kusanganiswa-kusanganiswa pakati pemitezo yemhuri yeMRNA ine hukama kunyangwe padanho rimwechete-bhesi-risingaenzaniswi.Iyi impedance-based biosensor inopa inoyevedza imwe nzira ye miRNA kutaura profiling uye inogona kugonesa kuvandudzwa kweinotakurika yakawanda miRNA profiling system.