Streptomycin sulfate CAS:3810-74-0 White powder
| Catalog Number | XD90361 |
| Product Name | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Molecular Formula | (C21H39N7O12)2·3H2SO4 |
| Molecular Weight | 1457.39 |
| Storage Details | 2 kusvika ku8 °C |
| Harmonised Tariff Code | 29412080 |
Chigadzirwa Specification
| Giredhi | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Kurasika paKuomesa | ≤7.0% |
| Assay | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Chitarisiko | Ichena kana inenge chena poda, yakanyanya kunyungudika mumvura, inenge isinganyunguriki mu ethanol. |
| Methanol | ≥0.3% |
| Streptomycin B | ≤3.0% |
| Colorimetric Test | ≥90.0% |
| Sulfuric Acid | ≤1.0% |
| Yakagadziriswa sodium sulphite yemukati | ≤0.4% |
Kunyange zvazvo etiology ye intervertebral disc degeneration isinganzwisisike, imwe nzira yekudzivirira iyi inogona kunge iri yekudzivisa apoptosis.Muchidzidzo chezvino, anti-apoptotic mhedzisiro ye carboxymethylated chitosan (CMCS) mu nucleus pulposus (NP) masero akaongororwa nechinangwa chekusimudzira disc cell kupona.Makonzo NP maseru aive ega uye akagadzirwa mu vitro, uye hydrogen peroxide (H2O2) yakashandiswa kuvaka iyo NP cell apoptosis modhi.Kushanda kwesero kwakaongororwa nesero kuverenga kit-8 assay.Chiyero chemasero eapoptotic chakaongororwa neannexin V-fluorescein isothiocyanate (FITC) uye propidium iodide (PI) kaviri kusvibisa ongororo, uye morphology yakaonekwa naHoechst 33342 staining.Iyo mitochondrial membrane inogona yeNP masero yakaongororwa nerhodamine 123 fluorescence staining.Reverse transcription (RT) -quantitative polymerase chain reaction (qPCR) yakaitwa kuyera mRNA mazinga einducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma (Bcl) ‑2, mhando II collagen uye aggrecan.Western blot ongororo yakaitwa kuti ione mapuroteni mazinga eNOS uye Bcl-2.Iyo annexin V-FITC/PI uye Hoechst 33342 yakasvibisa mhedzisiro yakaratidza kuti CMCS yakakwanisa kudzivirira NP masero kubva apoptosis nenzira inotsamira dosi.Rhodamine 123 staining yakajekesa kuti CMCS yakaderedza kukanganiswa kweiyo mitochondrial membrane inogona muH2O2-yakarapwa NP masero.Yakaderedzwa caspase-3 uye yakawedzera Bcl-2 chiitiko chakaonekwa muCMCS-yakarapwa NP maseru neRT-qPCR uye western blot kuongororwa.CMCS yakakurudzirawo kuwanda uye kuvanzika kwerudzi rwechipiri collagen uye aggrecan muH2O2-yakarapwa NP masero.CMCS yakaratidzwa kuti inoshanda mukudzivirira apoptotic cell death in vitro, ichiratidza zvingangobatsira zveiyi nzira yekurapa mukugadzirisa disc degeneration.
