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Cysteine ​​(Cys) inoita basa rakakosha mumaitiro mazhinji emuviri eukaryotic masero uye kuonekwa kwayo mumaseru ndiko kwakakosha.Zvisinei, glutathione (GSH), homocysteine, N-acetyl-L-cysteine ​​​​uye mamwe thiols anokanganisa zvikuru kuonekwa kweCys.Kunyanya, GSH inopindira zvakanyanya pakuonekwa kwemasero eCys (30-200 μM) nekuda kwekukwirira kwayo mukati memukati (1-10 mM).Mubasa iri, chidzitiro che-off-on fluorescent probe (HOTA) chekuonekwa kweCys chinoratidzwa.Iyi probe ine zvese zvakanakisa kunzwisiswa uye kunogutsa kusarudza kweserura Cys yekuona: nekuwedzera kwe200 μM Cys, iyo fluorescence intensity yeprobe (10 μM) yakakwidziridzwa 117-kupeta uye muganho wekuona wakaverengerwa kuita 13.47 μM, iyo yakaderera. kupfuura cellular Cys concentration;iyo probe zvakare yakasarudzwa yakaona 30-200 μM cysteine ​​​​pamusoro pe1-10 mM glutathione.Nekuda kweizvozvo, kuyedzwa kwekufungidzira kwesero kwakaitwa neprobe HOTA.Uyezve, migumisiro ye thiol-blocki ng uye GSH synthesis inhibiting miedzo yakasimbisa kuti intracellular emission inonyanya kutangira pakubatana pakati peCys neHOTA.