Bhakitiriya inonzi entomopathogenic, Xenorhabdus nematophila, inokonzera kudzivirirwa kwetwupukanana tunovavarira nekudzivisa phospholipase A(2) (PLA(2)) chiitiko.Munguva ichangopfuura, jini yakabatana nemuviri wePLA (2) yakaonekwa kubva muhupfu dzvuku beetle, Tribolium castaneum.Ichi chidzidzo chakagadzira iyi PLA (2) gene mune bhakitiriya kutaura vector kuti ibudise recombinant enzyme.Iyo recombinant T. castaneum PLA(2) (TcPLA(2)) yakaratidza maitiro ayo enzyme ine substrate concentration, pH, uye ambient tembiricha.Yayo biochemical maitiro anofananidzwa nerudzi rwekuvanzika kwePLA (2) (sPLA (2)) nekuti basa rayo rakavharwa nedithiothreitol (inoderedza agent ye disulfide bond) uye bromophenacyl bromide (yakananga sPLA (2) inhibitor) asi kwete nemethylarachidonyl. fluorophosphonate (yakananga cytosolic rudzi rwePLA (2)).Iyo X. nematophila culture muto yaive nePLA(2) inhibitory factor(s), iyo yaive yakawanda munhau dzakawanikwa padanho rekukura kwebhakitiriya.Iyo PLA (2) inhibitory factor (s) yaidzivirira kupisa uye yakatorwa mune zvese zvine aqueous uye organic zvikamu.Mhedzisiro yePLA (2)-inhibitory fraction pakudzivirira kweT. castaneum kwakaenzana zvakaenzana neyakakonzerwa nekudziviswa kweTcPLA (2) gene expression neRNA kupindira.
