A monoclonal antibody (mAb 8281) yakananga kune terminal β-galactose (βGal) yeglycosphingolipids (GSL) uye glycoproteins yakagadzirwa kubva kumakonzo akabaiwa ne lipid extract kubva kutsva acute lymphocytic leukemia (ALL) masero.Immuno-thin layer chromatography (ITLC) uye makwikwi emakwikwi ane akacheneswa asina kwaakarerekera eGSL zviyero, mahara shuga, uye synthetic neoglycoprotein yakaratidza mAb 8281 kuve yakasimba reactive neLacCer, GalCer uye Gal-β-O-(CH3)2S(CH3)2- CONH-(Gal-β-O-CETE) yakabatana nebovine serum albumin (BSA).Shuga yepeultimate yakaitawo basa mukusunga.Iyo antibody yanga isingaite nemacarbohydrates ane terminal αGal zvimiro uye zvisingaenderane terminal moieties.Indirect immunoperoxidase staining and flow cytometry ine mAb 8281 yakaratidza kusviba kwakanaka pamatishu akawanda, anosanganisira akatsetseka mhasuru, gastrointestinal mucosa, lymph node B masero uye monocytes.ITLC kuongororwa kweGSL kuumbwa kwetsva B cell neoplasms uchishandisa mAb 8281 yakasimbisa kuvapo kwelactosylceramide uye galactosylceramide mumaneoplasms ezvikamu zvakasiyana-siyana zvekusiyanisa.Nekuda kwehunyanzvi hwayo hwekupedzisira βGal carbohydrate residues, mAb 8281 inogona kubatsira mukuumbwa uye kushanda kwekuongorora kweGSL.
