4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Off-White Powder 98%
| Catalog Number | XD90011 |
| Product Name | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 30301.25 |
| Storage Details | -2ku-8°C |
| Harmonised Tariff Code | 29400000 |
Chigadzirwa Specification
| Mvura | <5% Karl Fische |
| Solubility | 1% muDMF yakajeka uye isina ruvara |
| Kuchena | Yemahara 4-Nitrophenol <200ppm |
| HPLC | >98% |
| Chitarisiko | Off-white powder |
Mechanistic njere muCa2+-inotsamira mhuri yealpha-mannosidases mudumbu remunhu symbiont.
Colonic mabhakitiriya, akaenzanisirwa neBacteroides thetaiotaomicron, anoita basa rinokosha mukuchengetedza hutano hwevanhu nekubatanidza mhuri huru dzeglycoside hydrolases (GHs) kuti dzishandise kudya kwepolysaccharides uye kugamuchira glycans sezvokudya.Kuwedzera kwemhuri yeGH kwakadaro kunoratidzirwa nemhuri ye23 GH92 glycosidases yakavharidzirwa neB. thetaiotaomicron genome.Pano isu tinoratidza kuti aya maalpha-mannosidases anoshanda kuburikidza neayo nzira yekubvisa kushandisa iyo inotambira N-glycans.Iyo yetatu-dimensional chimiro che maviri GH92 mannosidases inotsanangura mhuri yembiri-domain mapuroteni umo catalytic centre iri panzvimbo yedomasi interface, ichipa acid (glutamate) uye base (aspartate) rubatsiro kune hydrolysis muCa (2+) - kutsamira nzira.Iwo matatu-dimensional zvimiro zveGH92s zvakaomarara ane inhibitors zvinopa nzwisiso kune chaiyo, maitiro uye conformational rwendo rwe catalysis.Ca(2+) inoita basa rakakosha rekubatsira kutsveyamisa mannoside kubva panzvimbo yayo yepasi (4)C(1) chigaro chakanangana neshanduko.（Bibliography: Nat.Chem.Biol.6, 125-32, (2010)
Frontal affinity chromatography ye ovalbumin glycoasparagines pane concanavalin A-sepharose column.Chidzidzo chehuwandu hwekusungirirwa kweiyo lectin.
Kudyidzana kweSepharose 4B-immobilized concanavalin A (ConA) ine 10 glycoasparagines yakatorwa kubva kuovalbumin yakaongororwa zvakanyanya ne frontal affinity chromatography.Munzira iyi, mhinduro yemakhahydrates inoshandiswa nguva dzose kuConA-Sepharose column uye kunonoka kweElution front kunoyerwa separameter yesimba rekubatana.Iyo dissociation yenguva dzose (Kd) yega saccharide ine ConA inogona kutsanangurwa.Ongororo yekusungwa kwep-nitrophenyl-alpha,D-mannoside yakaratidza kuti inosunga zvimiro zveConA hazvishanduke zvakanyanya mushure mekusamira paSepharose 4B.Imwe neimwe yeovalbumin glycoasparagines yakanyorwa ne tritium neinoderedza methylation nzira yekuongorora.Kuenzanisa kweKd kukosha kwakawanikwa kwakaratidza kuti kusungirirwa kweConA kunosiyana zvakanyanya nekushomeka kwemaitiro akasiyana eiyo glycosyl cheni.Mhedzisiro yacho inoratidza kuti ConA inoziva chaiyo glycosyl cheni chimiro, Man alpha 1-6 (Munhu alpha 1-3) Murume, umo kanenge rimwe boka rehydroxyl paC-3 chinzvimbo cheC-6-yakabatana mannose inofanira kusununguka.
