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Monogalactopyranosides yefluorescein uye fluorescein methyl ester: synthesis, enzymatic hydrolysis ne biotnylated β-galactosidase, uye kutsunga kweshanduro yekuparadzira coefficient.

Fluorescein monoglycosides (d-galactopyranoside (FMG) uye d-glucopyranoside) uye yavo methyl ester (MFMG) yakagadzirirwa kubva kuacetobromoglucose/galactose uye fluorescein methyl ester mugoho rakanaka.Enzymatic hydrolysis experiments (uchishandisa biotinylated β-galactosidase) yegalacto derivatives yakaitwa uye kinetic parameters yakaverengwa.A 15-20 nguva kuwedzera kwesimba refluorescence yakaonekwa panguva yehydrolysis.Kuwedzera kwemutsara kwefluorescence kwave kuchicherechedzwa panguva pfupi uye yakaderera kusungirirwa kwe substrate, zvichiita kuti makomisheni aya abatsire uye anonzwisisika probes egalactosidases.Hukuru hweMichaelis-Menten yenguva dzose (Km) kukosha kweMFMG yakakwirira kupfuura iyo yeFMG ichikurudzira shanduko inogona kuitika yefluorogenic substrate.Km kukosha kwebiotinylated β-Gal ine FMG yakadzikira pane iyo enzyme yemuno.Uku kucherechedzwa kunoratidza yakakwirira substrate hukama hweiyo biotinylated enzyme mukuenzanisa neyemuno enzyme.Dudziro yediffusion coefficients yakayerwa, kune ese ari maviri fluorogenic substrates uye zvese zvigadzirwa, zvichishandisa fluorescence correlation spectroscopy.Transferal diffusion coefficients yefluorogenic substrates uye enzymatic hydrolysis zvigadzirwa zvakayerwa kuve zvakafanana, muhuwandu hwe3.5-4.5 × 10−10 m2 s-1.Saka kukwidziridzwa kana kudzikiswa kweiyo enzymatic kinetics nekuda kwekusiyana kwekushandura kwekufamba kwe substrate uye chigadzirwa hazvisi pachena.