Inoveli β-glucosidase gene, bgl1G5, yakagadzirwa kubva kuPhialophora sp.G5 uye yakabudirira kuratidzwa muPichia pastoris.Ongororo yekutevedzana yakaratidza kuti geni rine 1,431-bp yakavhurika yekuverenga furemu inokodha puroteni ye476 amino acids.Iyo yakatemerwa amino acid sequence ye bgl1G5 yakaratidza kuzivikanwa kwepamusoro kwe85% ine inozivikanwa β-glucosidase kubva kuHumicola grisea ye glycoside hydrolase mhuri 1. Kuenzaniswa nemamwe fungal shamwari, Bgl1G5 yakaratidza yakafanana optimal basa pa pH 6.0 uye 50 °C yaive 50 pa pH 5.0–9.0.Uyezve, Bgl1G5 yakaratidza kudziya kwakanaka pa50 ° C (6 h hafu yeupenyu) uye yakakwirira chaiyo chiitiko (54.9 U mg-1).Iyo K m uye V max maitiro akananga p-nitrophenyl β-D-glucopyranoside (pNPG) aive 0.33 mM uye 103.1 μmol min-1 mg-1, zvichiteerana.The substrate specificity assay yakaratidza kuti Bgl1G5 yainyanya kurwisa pNPG, isina simba pa p-nitrophenyl β-D-cellobioside (pNPC) uye p-nitrophenyl-β-D-galactopyranoside (ONPG), uye yakanga isina basa pane cellobiose.
