Isomeric bicyclo mbiri [4.1.0]heptane analogues yeglycosidase inhibitor galacto-validamine, (1R*,2S,3S,4S,5S,6S*) -5-amino-1-(hydroxymethyl)bicyclo[4.1.0]heptane -2,3,4-triol, yakagadzirwa mumatanho gumi nematatu kubva ku2,3,4,6-tetra-O-benzyl-D-galactose.Zviitiko zvekudzivirira zvemaamine maviri akaganhurirwa, nemaacetamides anoenderana nawo, akayerwa nemaenzayimu ekutengesa alpha-galactosidase kubva kubhinzi yekofi neE. coli.Basa remhuri yeglycosyl hydrolase GH27 enzyme (bhinzi yekofi) yakavharwa nemakwikwi ne1R, 6S-amine (7), kushamwaridzana kunosunga kwakaratidzwa neK (i) kukosha kwe0.541 microM.Iyo GH36 E. coli alpha-galactosidase yakaratidza kusasimba kwakanyanya kunosunga kudyidzana ne1R, 6S-amine (IC(50)= 80 microM).Iyo diastereomeric 1S, 6R-amine (9) yakasungwa zvisina simba kune ese ari maviri galactosidases, (kofi bhinzi, IC(50)= 286 microM) uye (E. coli, IC(50)= 2.46 mM).
