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Mhedzisiro ye detergents paP-glycoprotein atpase chiitiko: misiyano mukukanganisa kwebasal uye verapamil-inotsamira mabasa.

P-glycoprotein (P-gp), plasma membrane glycoprotein inobatanidza ne-multidrug resistance phenotype, inokonzera ATP-inotsamira efflux yemishonga yakasiyana-siyana yemamphiphilic.Tichishandisa membrane vesicles yakagadzirwa kubva kune multidrug resistant cell line DC-3F/ADX, takadzidza kuvhiringika kweiyo basal (kureva mukushaikwa kwemushonga) uye verapamil-inotsamira P-gp ATPase zviitiko zvinokonzerwa neakasiyana detergents, pakusa-solubilizing, uyewo pakusolubilizing, concentrations.Iyo inofambira mberi membrane solubilization nekuwedzera detergent concentration yakatariswa nekupararira kwechiedza uye centrifugation kuyedza.Nokuda kwekusagadziriswa kwemafuta ekuchenesa, ese akaedzwa detergents kunze kweDOC yakakonzera kuvharidzirwa kwechikamu cheP-gp ATPase chiitiko, icho chakanga chisingaenderani nehuwandu hwemhando dzakasiyana-siyana dzakaedzwa dzakabatanidzwa mu membranes.Ongororo yeVerapamil-induced P-gp activation inoratidza kuti P-gp ATPase chiitiko chakagadziridzwa zvakasiyana neakasiyana ma detergents pane asiri-solubilizing concentrations.Saka, kudyidzana kwakananga pakati peP-gp uye detergents kunowanzoitika pane kuvhiringika kwepasi rose membrane.Mushure mekugadzirisa nemishonga yakasiyana-siyana yakaedzwa, basa reP-gp ATPase rakange rakadzivirirwa zvachose, kunze kwekuvapo kweCHAPS iyo yakakwanisa kuchengetedza chiitiko ichi pamwero wakaenzaniswa neyakayerwa mumambure ekuzvarwa.Nekudaro, iyo verapamil-induced P-gp ATPase activation yakarasika panguva yeP-gp solubilization neCHAPS, asi yakapora mushure mekunatswa kweCHAPS pazasi kwayo yakakosha micellar concentration.Aya maonesheni anoratidza kudyidzana chaiko pakati peP-gp neCHAPS mamorekuru mukati memicelles akasanganiswa.Pakati pese, data yedu inopupurira kupindirana kwakananga P-gp / detergents inopindirana nenzvimbo yenzvimbo dzekutakura zvinodhaka paP-gp transmembrane domains.