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MES Buffer Inobata Arabidopsis Root Apex Zonation uye Root Kukura nekudzvanyirira Superoxide Generation mu Root Apex

Muzvirimwa, kukura kwemidzi uye midzi mvere kunodzorwa neiyo yakanaka cellular control yepH uye reactive oxygen marudzi (ROS).MES, 2-(N-morpholino) ethanesulfonic acid seimwe yeGood's buffers yakashandiswa zvakanyanya kubhafa yepakati, uye inofungidzirwa kuti inokodzera kukura kwechirimwa neconcentration pa0.1% (w/v) nekuti buffer kugona MES inotangira pH 5.5-7.0 (yeArabidopsis, pH 5.8).Nekudaro, mishumo mizhinji yakaratidza kuti, mumasikirwo, midzi inoda akasiyana pH kukosha pamusoro peiyo chaiyo midzi apex zones, zvinoti meristem, transition zone, uye elongation zone.Kunyangwe chokwadi chekuti midzi inogara ichikura pane midhiya ine buffer molecule, zvishoma zvinozivikanwa nezve kukanganisa kweMES pakukura kwemidzi.Pano, isu takatarisa mhedzisiro yeakasiyana kuwanda kweMES buffer tichishandisa midzi inokura yeArabidopsis thaliana.Mhedzisiro yedu inoratidza kuti 1% yeMES yakadzivirira zvakanyanya kukura kwemidzi, nhamba yemvere dzemidzi uye kureba kwemeristem, nepo 0.1% yakasimudzira midzi yekukura uye midzi apex nzvimbo (nzvimbo inotenderera kubva pamudzi weti kusvika kunzvimbo yekuchinja).Uyezve, chizvarwa che superoxide mumudzi apex chakanyangarika pa1% yeMES.Mhedzisiro iyi inoratidza kuti MES inovhiringidza yakajairika midzi morphogenesis nekushandura iyo ROS homeostasis mumudzi apex.