Beta-glucosidase gene (bgl3) kubva kuStreptomyces sp.QM-B814 (American Type Culture Collection 11238) yakaumbwa nekushanda kwekuwedzera kwe beta-glucosidase-negative mutant yeStreptomyces lividans.Chimiro chekuverenga chakavhurika che1440 nucleotides encoding polypeptide ye479 amino acids yakawanikwa nekutevedzana.Protein yakavharidzirwa (Bgl3) inoratidza kufanana kwakakura (kupfuura 45% kuzivikanwa) ne beta-glycosidases kubva kumhuri-1 glycosyl hydrolases.Iyo enzyme yakaumbwa, yakacheneswa zvichitevera kunaya kweammonium sulphate uye nhanho mbiri dzechromatographic, ndeye monomeric ine molecular mass 52.6 kDa, sezvakatemwa ne mass spectrometry, uye isoelectric point yepI 4.4.Iyo enzyme inoita kunge beta-glucosidase ine yakafara substrate chaiyo, inoshanda pane cellooligomers, uye inoita transglycosylation reactions.Iyo inofungidzirwa inooneka Km kukosha kwep-nitrophenyl-beta-D-glucopyranoside uye cellobiose ndeye 0.27 mM uye 7.9 mM, zvichiteerana.Iyo Ki values yeglucose uye delta-gluconolactone, uchishandisa p-nitrophenyl-beta-D-glucopyranoside se substrate, ndeye 65 mM uye 0.08 mM, zvichiteerana.Iyo enzyme yakacheneswa ine pH optimum yepH 6.5 uye tembiricha yakakwana yekuita i50 madhigirii.
