Biosynthesis yeproteoglycans uye glycosaminoglycans pamberi pep-nitrophenyl-xyloside yakadzidzwa pachishandiswa gonzo rekutanga ovarian granulosa cell tsika system.Kuwedzerwa kwep-nitrophenyl-xyloside muchitokisi chetsika yakakonzera kuwedzera kwe 700% ye [35S] sulfate incorporation (ED50 pa 0.03 mM) mumacromolecules, iyo yaisanganisira yemahara chondroitin sulfate cheni dzakatangwa pa xyloside uye chizvarwa cheproteoglycans.Cheni dzemahara chondroitin sulfate dzakatangwa pa xyloside dzakapotsa dzavharirwa mukati mepakati.Iyo molecular saizi yechondroitin sulfate maketani yakadzikira kubva ku40,000 kusvika ku21,000 sezvo yakazara [35S] sulfate incorporation yakasimbiswa, zvichiratidza kuti kuwedzeredzwa synthesis ye chondroitin sulfate yakakanganisa nzira yakajairika yeglycosaminoglycan chain termination.Biosynthesis yeheparan sulfate proteoglycans yakaderedzwa neinenge 50%, zvichida nekuda kwekukwikwidza pamwero weUDP-shuga precursors.[35S]Sulfate incorporation yakavharwa nekuwedzerwa kwe cycloheximide nehafu yekutanga yenguva inenge 2 hr pamberi pe xyloside, apo iyo pasina xyloside yaiva inenge 20 min.Musiyano uyu ungangoratidza chiyero chekuchinja kweglycosaminoglycan synthesizing kugona zvachose.Huwandu hwekuchinja kweglycosaminoglycan synthesizing capacity inocherechedzwa mumaovarian granulosa masero yaive ipfupi pane iyo yakaonekwa mumachondrocytes, zvichiratidza hutongi hwehukama hweproteoglycan biosynthetic chiitiko mune yakazara metabolic chiitiko chemasero.
