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Huchapupu huchangoburwa hunotsigira basa riri kubuda re β-nicotinamide adenine dinucleotide (β-NAD(+)) senovhero neurotransmitter uye neuromodulator mu peripheral nervous system -β-NAD(+) inoburitswa mutsinga-inopfava mhasuru gadziriro uye adrenal chromaffin masero. nenzira inofananidzira neurotransmitter.Parizvino hazvizivikanwe kana izvi zvine chokwadi kune CNS.Tichishandisa diki-chamber superfusion assay uye high-sensitivity high-pressure liquid chromatography techniques, tinoratidza kuti high-K(+) kukurudzira kwemakonzo forebrain synaptosomes inomutsa kufashukira kwe β-NAD(+) , adenosine 5'-triphosphate, uye yavo. metabolites adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate, adenosine, ADP-ribose (ADPR) uye cyclic ADPR.Iyo yepamusoro-K (+) -yakadzvinyirirwa mafashama e β-NAD(+) inodzikiswa nekutsemuka kweSNAP-25 ine botulinum neurotoxin A, nekuvharirwa kweN-mhando voltage-inotsamira Ca (2+) chiteshi ine ω-conotoxin GVIA. , uye nekudzivirirwa kweproton gradient yesynaptic vesicles ine bafilo mycin A1, zvichiratidza kuti β-NAD(+) inogona kuburitswa kuburikidza nevesicle exocytosis.Ongororo yekuMadokero inoratidza kuti CD38, puroteni inoshanda yakawanda inogadzirisa β-NAD(+) , iripo pa synaptosomal membranes uye mu cytosol.Itact synaptosomes inosvibisa β-NAD(+) .1,N (6) -etheno-NAD, fluorescent analog ye β-NAD (+) , inotorwa ne synaptosomes uye kutora uku kunoderedzwa nechokwadi β-NAD (+) , asi kwete ne connexin 43 inhibitor Gap 27. Mune cortical neurons kushandiswa kwenzvimbo kwe β-NAD(+) kunokonzera kukurumidza Ca (2+) inopera, zvichida nekuda kwekuwanda kwe extracellular Ca (2+) .Naizvozvo, rat brain synaptosomes inogona kushingaira kusunungura, kuderedza uye kutora β-NAD (+) , uye β-NAD (+) inogona kukurudzira postsynaptic neurons, zvose zvinodiwa kuti chinhu chionekwe semumiriri neurotransmitter muuropi.