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Polymer brush layers yakavakirwa pablock copolymers epoly(oligo(ethylene glycol) methacrylate) (POEGMA) uye poly(glycidyl methacrylate) (PGMA) akaumbwa pasilicon wafers nema activators anogadzirwa neelectron transfer atomu transfer radical polymerization (AGET ATRP).Mhando dzakasiyana dzebiomolecule dzinogona kusanganisirwa kune aya mabhurashi akaturikidzana nekuita kwePGMA epoxide mapoka nemapoka emaamino mubiomolecule, nepo POEGMA, iyo inoramba nonspecific protein adsorption, inopa antifouling nharaunda.Mapaziro airatidzwa nemvura yekubata kona, ellipsometry, uye Fourier inoshandura infrared spectroscopy (FTIR) kusimbisa maitiro ekugadzirisa.Kupatsanurwa kwechikamu chezvivharo zvecopolymer muzvikamu zvakaonekwa neAFM.Mhedzisiro yezvivakwa zvepasi paprotein conjugation yakaongororwa pachishandiswa nzira dzeradiolabeling.Zvakaratidzwa kuti mapeji ane POEGMA akaturikidzana aishingirira mapuroteni, nepo huwandu hweprotein hwakabatanidzwa kune diblock copolymer yakagadziridzwa nzvimbo yakawedzera sesed nekuwedzera kwePGMA layer.Chiitiko che lysozyme chakabatanidzwa pamusoro chinogona zvakare kudzorwa nekusiyanisa ukobvu hweiyo copolymer layer.Apo biotin yakabatanidzwa kune block copolymer grafts, nzvimbo yacho yakaramba ichipikisa kune nonspecific protein adsorption asi yakaratidza kusungirirwa kwakananga kweavidin.Izvi zvimiro, kureva, yakanyatsodzorwa huwandu uye chiitiko che conjugated biomolecules uye chaiyo yekudyidzana neinongedzo biomolecules inogona kushandiswa kuvandudza kwechiratidzo-ku-ruzha reshiyo mumasensor application.Zvakawanda, nzvimbo dzakadai dzinogona kubatsira senge biological recognition zvinhu zvemhando yepamusoro kune inoshanda biomaterials.