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Bicine izwitterionic amino acid buffer, inoshanda mupH 7.6-9.0 renji (pKa ye8.26 pa25°C).Yakakurudzirwa buffer ye yakaderera tembiricha biochemical basa.Bicine inoshandiswa kugadzirira yakagadzikana substrate mhinduro ye serum guanase kutsunga.Kushandiswa kwebicine mune yakatetepa layer ion exchange chromatography nzira yekugadziriswa kweprotein yakaburitswa.Bicine yakashandiswa mu peptide uye protein crystallization.Chidzidzo che kinetic che quaternary transition-state analogue complex ye creatine kinase yakashandiswa bhicine mukuita buffer.A multiphasic buffer system yeSDS-PAGE yemapuroteni uye peptides iyo inosanganisira bicine yakatsanangurwa.

Single alveolar madziro akaiswa kune kureba-tension zvidzidzo mu saline uye Bicine (0.2 M) inoenda kunowedzera kuora mukushushikana kwetishu (TTD).Isu takaongorora mhedzisiro yemhinduro dzakasiyana pane iyi TTD uye takatsvaga shanduko dzinoenderana mune yekupedzisira.Lung parenchyma yakatsemurwa kune imwechete alveolar madziro (30 X 30 X 150 microns) muphosphate-buffered saline (0.15 M).Yakaendeswa kune yekugezesa-kurebesa yekugezesa, iyo tishu yakanyudzwa muBicine, saline, yakasimbiswa Hank's solution, 0.25% Alcian yebhuruu mumunyu, kana sodium dodecyl sulfate solution, kwenguva dzakasiyana.Yakatenderedzwa kuburikidza nekuwedzerwa kwakapihwa nesimba repamusoro rakayerwa nekufamba kwenguva, aya matishu akaiswa mubuffered glutaraldehyde/tannic acid uye akagadziridzwa maelectron microscopy.Single alveolar madziro akanyudzwa musaline kana Bicine airatidza kufambira mberi kweTTD.Vacuoles kana nzvimbo dzakaonekwa muinterstitium iyo nekusagadzikana kweserura kwakafambira mberi neTTD.Kuonekwa mukati me0.3 h, shanduko dzakafambiswa zvakanaka pa 0.6 h.Mu sodium dodecyl sulfate (70 mM), zvisinei, pakanga pasina TTD uye muchimiro pakanga pasina interstitium, iine membrane yepasi chete uye mapuroteni ane fibrous akasara.Mune yakasimbiswa Hank's solution kana 0.25% Alcian blue iyo interstitial matrix, cell morphology uye kushushikana kwetishu zvakachengetwa zvakanaka kwe1 h.Ongororo iyi inoratidza kuti leaching ye interstitial matrix inoitika mu saline kana Bicine, uye matrix akasimba akakosha pakuchengetedza tension.