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Nhanganyaya: BSA nderimwe remapuroteni anonyanya kushandiswa mumarabhoritari ebiochemical, uye kukosha kwayo kunogona kufuratirwa mukuyedza nekuti yakawandisa uye yakawandisa.Bovine serum albumin (BSA), inozivikanwawo sechikamu chechishanu, iglobulin mubovine serum ine 583 amino acid zvisaririra, ine molecular huremu hwe66.430kDa uye isoelectric point ye4.7.BSA ine huwandu hwakawanda hwemashandisirwo muzvidzidzo zvebiochemical, senge Blocking agent mumadokero blots.

Kushandisa: Bovine Serum Albumin (BSA), inozivikanwawo sechikamu chechishanu, iglobulin mubovine serum, ine 607 amino acid residues, uye ine zvakawanda zvakasiyana-siyana zvekushandisa mukuongorora kwebiochemical.Bovine serum albumin inowanzoshandiswa seyakagadzikana mukugadzirisa mhinduro uye mhinduro yekugadzirisa yekudzivirira ma enzymes kana ma enzymes akagadziridzwa kudzikamisa basa re enzyme uye kudzivirira kuparara kwe enzyme uye isiri-yakananga adsorption.

Basa: BSA inowanzo shandiswa seyakagadzika mugadziriso yekuchengetera uye mhinduro mhinduro yekurambidza ma enzymes kana ma enzymes akagadziridzwa, nekuti mamwe ma enzymes haana kugadzikana kana ane yakaderera chiitiko pane yakaderera.Mushure mekuwedzera BSA, inogona kuita basa re "kudzivirira" kana "mutakuri", uye basa rema enzymes mazhinji rinogona kuvandudzwa zvakanyanya mushure mekuwedzera BSA.MaEnzymes asingade kuwedzerwa kweBSA kazhinji haakanganisike nekuwedzera kweBSA.Kune yakawanda substrate DNA, BSCA inogona kuita kuti kugaya kuwedzere kuzara, uye inogona kuwana kudzokorora kucheka.Pa 37 ° C, apo kugadzirisa kwekudya kunodarika 1 h, BSA inogona kuita kuti enzyme iwedzere kugadzikana, nokuti mukugadzirisa buffer pasina BSA, ma enzyme akawanda anodzivisa anogona kurarama pa 37 ° C kwe10 ~ 20min kana kunyange nguva shoma..Kusiyana neizvi, BSA inogona kusunga simbi ions uye mamwe makemikari mubuffer kana substrate DNA inotadzisa basa rekurambidza endonucleases.

Zvishandiso: Yakajairwa giredhi bovine serum albumin (BSA, StandardGrade), iyo inogona kusangana nezvinodiwa zveakawanda maitiro ekuedza, senge immunoblocking agent, tissue cell (microbial mhuka uye zvipembenene masero, etc.) tsika chikafu uye tsika zvikamu, protein / enzyme kugadzikana. reagents uye mapuroteni quantification zviyero.Diagnostic giredhi bovine serum albumin (BSA, DiagnosticGrade) inogona kusangana nezvakawanda zvechiyedzo zvinodiwa, senge immunoblocking agent, protein/enzyme stabilizer, diluent, carrier uye protein standard.Uye zvakare, inogona zvakare kushandiswa kune immunoassays, sero tsika uye hybridization miedzo inoda kunzwisiswa kwakanyanya.