PMSF Cas: 329-98-6 98.0% white crystalline powder Phenylmethanesulfonyl fluoride (PMSF)
| Catalog Number | XD90250 |
| Product Name | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Molecular Formula | C7H7FO2S |
| Molecular Weight | 174.1927 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 29049900 |
Chigadzirwa Specification
| Assay | ≥98.0% HPLC |
| Chitarisiko | white crystalline powder |
PMSF is irreversible serine/cysteine protease inhibitor inowanzoshandiswa mukugadzirira kwesero lysates.
In vitro zvidzidzo: PMSF (2 mM) inhibited carbachol-inokurudzira inositol phosphate kuunganidza chete 15% -19% pamberi peLi +.Kudzivirirwa kwe phosphoinositide kutendeuka nePMSF kunokonzerwa nenhanho imwe kana kupfuura inotevera kuparara kwephosphoinositides [1].PMSF inhibits acylation yeinositol residues yeGPI yepakati muropa reT. brucei.PMSF inhibits glycolipid C kuumbwa asi kwete fatty acid remodeling in vitro.PMSF inhibits kuwedzera kweGPI acylation uye ethanolamine phosphatase mu procyclic trypanosomes, asi kwete muHela masero [2].
In vivo zvidzidzo: PMSF (0.1 mL / 10 g b.wt, ip) yakabudisa antinociception, sezvinoratidzwa nedosi-inopindura kuwedzera mu% MPE mumuswe-flick latency assessment, asi yakakundikana kubudisa pachena dose-responsive motor inhibition.Makonzo akagamuchira intraperitoneal jekiseni yePMSF yakaratidza cannabinoid mhedzisiro inosanganisira antinociception, hypothermia uye kusafamba ne ED50 kukosha kwe86, 224 uye 206 mg / kg, zvichiteerana.PMSF (30 mg/kg) pretreatment inowedzera mhedzisiro yeanandamide pamhinduro dzemuswe-flick (antinociception), basa relocomotor uye kufamba ne5-fold, 10-fold uye 8-fold, zvichiteerana [3].
Kuedza kwemhuka: Makonzo eICR anorema 18 kusvika 25 g akashandiswa muyedzo.PMSF yakanyungudutswa mumafuta eSesame uye inoshandiswa intraperitoneally muhuwandu hwe0.1 mL / 10 g b.wt.Nguva dzose shandisa PMSF 10 maminetsi isati yasvika intravenous anandamide kana jekiseni remotokari.Mbeva dzakajairirwa muimba yekuongorora husiku pasina kukanganiswa kwechikafu kana mvura.Kutevera intravenous anandamide kana kutungamirirwa kwemotokari, mhuka imwe neimwe yakaongororwa sezvinotevera: 5 maminitsi emuswe-flick latency (antinociceptive) mhinduro uye 5 kumaminitsi e15 nokuda kwekuita (motor) basa;kana 5 maminetsi epakati (rectal) tembiricha uye mhete Immobilization (catalepsy) kwemaminetsi mashanu kusvika gumi.
