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Yakashandiswa kugadzira CAPS buffer, zwitterionic buffer inobatsira muhuwandu hwepH 7.9-11.1.CAPS buffer inoshandiswa zvakanyanya muWestern uye immunoblotting kuyedza pamwe neprotein sequencing nekuzivikanwa.Inoshandiswa mu electrotransfer yeprotein kuPVDF (sc-3723) kana nitrocellulose membranes (sc-3718, sc-3724).Iyo yakakwirira pH yeiyi buffer inoita kuti ibatsire pakufambiswa kwemapuroteni ane pI> 8.5.uye kushoma reactivity nema enzymes kana mapuroteni, mashoma emunyu mhedzisiro.

Mu capillary zone electrophoresis kumhanyisa kwe electrophoretic yeion kunodzikira sezvo kudzika kwemushonga we electrolyte wekumashure kuchiwedzerwa.Izvi zvinokonzerwa nekuchinja kweiyo electrophoretic mobility yeion (muep) pamwe chete nekushandurwa kwesimba remambure rinoibata, kureva simba remagetsi rinoshanda (Eeff).Iyo electrophoretic mobility yeion inoshandurwa kuburikidza nekuchinja kweabsolute viscosity ye electrolyte mhinduro uye shanduko muhukuru hwakagadziriswa hweion.Eeff inoshandurwa zvakanyanya nekuchinja kwehukuru hwekuchaja asymmetry athari uye electrophoretic athari, izvo zvese zvinodzosa kufamba kwemaion.Muchidzidzo ichi, matatu-marker technique yakashandiswa kudzidza mhedzisiro yemashure electrolyte concentration (0.02-0.08M 3--[cyclohexylamino] -1-propanesulfonic acid uye counter ion (Li, Na, K, uye Cs) paEeff. Zvakaonekwa kuti kuungana kwemaelectrolyte ekumashure kunokanganisa Eeff zvakanyanya uye kuti Eeff inosvika paE apo kusangana kweelectrolyte yekumashure kunosvika pazero. Iyoni yekaunda yakave nemhedzisiro diki paEeff: kukura kweiyo hydrated yeiyoni yekaunda yakawedzera. , Eeff yakadzikira.Nzvimbo yezvicherechedzo zvitatu yakaratidza kuva inoshanda pakutema kwakadaro.