D-Luciferin Cas: 2591-17-5 99% Off-white kusvika yellow powder nbsp BEETLE LUCIFERIN
| Catalog Number | XD90248 |
| Product Name | D-Luciferin |
| CAS | 2591-17-5 |
| Molecular Formula | C11H8N2O3S2 |
| Molecular Weight | 280.323 |
| Storage Details | -15 kusvika -20 °C |
| Harmonised Tariff Code | 29342080 |
Chigadzirwa Specification
| Mvura Yemukati | Max.2.0% |
| Turbidity | Max 2.0 NTU |
| Specific optical rotation | -36 kusvika -32 |
| Chitarisiko | Kuchena-kuchena kusvika kuhupfu yeyero |
| Purity HPLC | Zvishoma 99% |
| Molar extinction coefficient | min 17900 L/(mol cm) |
Nhanganyaya: D-luciferin is substrate for adenosine triphosphate (ATP)-inotsamira bioluminescence reaction.Nheyo ye bioluminescence ndeyokuti luciferin yakasvibiswa ne luciferase pamberi peATP uye oksijeni.Mushonga wekuita makemikari unotevera: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Chiedza.
Mechanism of action: Nzira yekuita kweD-luciferin ndeyokuti pasi pechiito cheATP uye luciferase, luciferin (substrate) inogona kuiswa oxidized kuti ibudise chiedza.Kana luciferin iri kuwandisa, nhamba yemafotoni anogadzirwa anonyatsoenderana nehuwandu hwe luciferase.
Kushandisa: D-luciferin is substrate yeadenosine triphosphate (ATP)-inotsamira bioluminescence reactions.Iyo bioluminescence reaction ye luciferin / luciferase inowanzoshandiswa pakuona kweATP, metabolites inogona kuchinjwa kuva ATP (yakadai se AMP, ADP, cAMP), uye ma enzymes anogona kugadzira ATP (yakadai se creatine kinase, nezvimwewo), saka iyo bioluminescence reaction inogona kushandiswa.Inoshanda pakuonekwa kwehuwandu hwakawanda hwezvinhu zvipenyu.
Biological Activity: D-Luciferin (Firefly luciferin) inonzi substrate yakakurumbira yebioluminescence pamberi peATP inoshandiswa mu luciferase-based bioluminescence imaging uye cell-based high-throughput screening.
In vitro zvidzidzo: D-luciferin inopindirana ne luciferase, ATP uye oksijeni kuti ibudise chiedza, iyo inotaridzirwa nefirimu yemifananidzo yakajeka kuti ione masoja e-alkaline phosphatase-bound.
In vivo zvidzidzo: kushandiswa kweD-luciferin substrates uye firefly luciferase inochengetedza tumor-host immune kupindirana mune immunocompetent mouse modhi ine ovarian cancer, sezvo bioluminescence zvirongwa zvinonyanya kuona bundu kukura pane kurema kwemuviri mirairo.
