dCMP, 2'-Deoxycytidine 5'-monophosphate, yemahara asidhi CAS: 1032-65-1
| Catalog Number | XD90549 |
| Product Name | dCMP, 2'-Deoxycytidine 5'-monophosphate, isina asidi |
| CAS | 1032-65-1 |
| Molecular Formula | C9H14N3O7P |
| Molecular Weight | 307.20 |
| Storage Details | -15 kusvika -20 °C |
| Harmonised Tariff Code | 29349990 |
Chigadzirwa Specification
| Chitarisiko | Solid |
| Assay | 99% |
Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) ine simba chaiyo inhibitor yehepatitis C virus (HCV) RNA synthesis muHuh-7 replicon masero.Kuti inhibisa iyo HCV NS5B RNA polymerase, PSI-6130 inofanirwa kuve phosphorylated kune iyo 5'-triphosphate fomu.Iyo phosphorylation yePSI-6130 uye inhibition yeHCV NS5B yakaongororwa.Iyo phosphorylation yePSI-6130 ne recombinant human 2'-deoxycytidine kinase (dCK) uye uridine-cytidine kinase 1 (UCK-1) yakayerwa nekushandisa yakabatanidzwa spectrophotometric reaction.PSI-6130 yakaratidzwa kuva substrate yekucheneswa dCK, ine Km ye81 microM uye kcat ye 0.007 s-1, asi yakanga isiri substrate yeUCK-1.PSI-6130 monophosphate (PSI-6130-MP) yaive yakanyatso phosphorylated kune diphosphate uye yakazotevera kune iyo triphosphate neinodzokorodza munhu UMP-CMP kinase uye nucleoside diphosphate kinase, zvichiteerana.Iyo inhibition yemhando yesango uye yakashandurwa (S282T) HCV NS5B RNA polymerases yakadzidzwa.Iyo steady-state inhibition constant (Ki) yePSI-61 30 triphosphate (PSI-6130-TP) ine mhuka yemusango enzyme yaive 4.3 microM.Migumisiro yakafanana yakawanikwa ne 2'-C-methyladenosine triphosphate (Ki = 1.5 microM) uye 2'-C-methylcytidine triphosphate (Ki = 1.6 microM).NS5B ine S282T mutation, inozivikanwa kupa kuramba 2'-C-methyladenosine, yakavharwa nePSI-6130-TP zvine hungwaru semhando yemusango.Kubatanidzwa kwePSI-6130-MP muRNA yakagadziriswa neyakacheneswa NS5B RNA polymerase yakakonzera kupera kweketani.
