Dithizone CAS: 60-10-6 80% Black blue powder
| Catalog Number | XD90464 |
| Product Name | Dithizone |
| CAS | 60-10-6 |
| Molecular Formula | C13H12N4S |
| Molecular Weight | 256.33 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 29309098 |
Chigadzirwa Specification
| Kurasika paKuomesa | ≤5% |
| Chitarisiko | Black blue powder |
| Assay | 80% |
| Residue paIgnition | ≤0.2% |
Mesenchymal stem masero (MSCs) anonzi akanakisa sosi yekugadzirwa kweanogona transplantable surrogate β cell.Murine embryonic mesenchymal progenitor cell line C3H10T1/2 yave kuzivikanwa semuenzaniso weMSCs, nekuda kwekugona kwayo kusiyanisa mitsara yakawanda.Chinangwa chechidzidzo ichi chaiva chekuongorora kana masero eC3H / 10T1 / 2 ane mukana wekusiyanisa masero anogadzira insulini (IPCs).Pano, takaongorora uye tikaenzanisa in vitro mutsauko wemakonzo MSCs uye C3H10T1/2 maseru kuita IPCs.Mushure mekunge masero aita IPC kusiyanisa, kutaura kwekusiyanisa mamaki kwakaonekwa ne immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR (qRT-PCR) uye Western blotting.Iyo insulin secretion yakaongororwa ne enzyme-yakabatana immunosorbent assay (ELISA).Uyezve, aya masero akasiyaniswa akaiswa mu streptozotocin-induced diabetic mice uye mabasa avo ehupenyu akaedzwa mu vivo.Ichi chidzidzo chinoshuma nzira ye2-nhanho yekugadzira IPCs kubva kuC3H10T1/2 maseru.Pasi pemamiriro chaiwo ekugadzirisa kwemazuva 7-8, masero eC3H10T1 / 2 akaumba matatu-dimensional spheroid miviri (SBs) uye yakavanza insulini, nepo chizvarwa cheIPCs chinobva kumakonzo MSCs chaida nguva yakareba (kupfuura mavhiki maviri).Uyezve, aya maIPC akatorwa kubva kuC3H10T1 / 2 masero akaiswa mumakonzo ane chirwere cheshuga uye anovandudza basal glucose, uremu hwemuviri uye yakaratidza yakajairika glucose kushivirira bvunzo.Chidzidzo chezvino chakapa yakapusa uye yakatendeka in vitro modhi yekuenderera mberi nekuongorora maitiro ari pasi peIPC kusiyaniswa kweMSCs uye cell replacement therapy yechirwere cheshuga.
