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Mycobacterium tuberculosis inogona kuramba ichienderera kwemakumi emakore mumunhu.Nzira dzakasimba dzekupindura dzinosanganisira inorganic polyphosphate [poly(P)], iyo inogadzirwa uye hydrolyzed ne polyphosphate kinase (PPK) uye exopolyphosphatase (PPX), zvichiteerana, inofungidzirwa kuti inobata basa rakakosha rekutonga mukuramba kwebhakitiriya.Isu tinoratidza pano kuti M. tuberculosis poly(P) kuunganidzwa kunobatanidzwa kwenguva pfupi nekudzivirira kukura kwebacillary.Isu tinozivisawo M. tuberculosis Rv1026 sechinyorwa cheexopolyphosphatase chine hydrolytic chiitiko chinopesana ne-long-chain poly(P).Tichishandisa tetracycline-inducible expression system kurovera pasi kutaura kweRv1026 (ppx2), takaona kuti M. tuberculosis poly(P) kuunganidzwa kunotungamirira kukudzikira kukura uye kuderedza kubatwa ne isoniazid, kuwedzera kupikisa kupisa uye acid pH, uye kunowedzera kurarama kwemukati memasero. panguva yehutachiona hwemacrophage.Nekutapurirana electron microscopy, iyo ppx2 knockdown strain yakaratidza kuwedzera kwesero madziro makubvu, ayo aienderana neyakaderedzwa sero madziro kupenya kune hydrophilic mishonga pane kupinza zvinodhaka efflux pombi kana kuchinjwa biofilm kugadzirwa zvine chekuita neiyo isina chinhu vector control.Transcriptomic uye metabolomic analysis yakaratidza metabolic downshift yeppx2 knockdown inoratidzwa nekuderedzwa kunyorwa uye kududzira uye kuderera kwemazinga eglycerol-3-phosphate.Muchidimbu, poly(P) inoita basa rakakosha muM. tuberculosis kukura kwekudzivirira uye metabolic downshift uye inobatsira kushivirira kwemishonga inorwisa mabhakitiriya kuburikidza nekuchinja kwesero kupenya kwemadziro.Mapinduriro akaomarara, anosanganisira anodzora mamorekuru inorganic polyphosphate [poly(P)] uye (p. ) ppGpp, inotendwa kuti inoyananisa kuenderera mberi kweMycobacterium tuberculosis.Muchidzidzo ichi, takaona riini enzyme (Rv1026, PPX2) inotarisira hydrolyzing refu-cheni poly(P).A genetically engineered M. tuberculosis strain in the ppx2 gene yakaratidza kuwedzera kwepoly(P) mazinga, ayo aibatanidzwa nekutanga kwekukura kwebhakitiriya kusungwa uye kuderedza susce ptibility kumushonga wekutanga isoniazid, pamwe nekuwedzera kupona kwebhakitiriya panguva yekusangana nekushungurudzika. mamiriro uye mukati macrophages.Zvichienderana nekunetseka kwekutonga, iyo mutant yakaratidza kuwedzera kukora kwesero madziro uye kuderedzwa kwezvinodhaka permeability.Global gene expression uye ongororo yemetabolite yakaratidza kuderedzwa kwekutaura kwechinyorwa uye chekushandura muchina uye shanduko mukushandiswa kwekabhoni sosi.Muchidimbu, kudzorwa kwepoly(P) chiyero kwakakosha pakuramba kuumbwa muM. tuberculosis.