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Chimwe chezvikonzero zvinodzivirira kushandiswa kwekiriniki kwemishonga yekuvandudza kune degenerative cartilage zvirwere ndiyo yakakodzera masero.Chondrocytes, iyo chete sero rudzi rwecartilage, yakakura mu vitro pasi pemamiriro etsika kuti iwedzere maseru nhamba inorasikirwa nephenotype yavo pamwe nekukwanisa kugadzira hyaline cartilaginous tishu.Muchidzidzo ichi tinoona kuti serum- uye kukura-isina-matatu-dimensional (3D) tsika yetsika inodzorera kukwanisa kwechondrocytes yakapfuura kuti iite cartilage tissue in vitro, chirongwa chinosanganisira sox9.Bovine articular chondrocytes yakapfuura kaviri kusvika bvumira kuwedzera kwenhamba yenhamba (P2) uye yakasimwa pahupamhi hwepamusoro pa 3D collagen-type-II-coated membranes mune high glucose content media inowedzerwa ne insulin uye dexamethasone (SF3D).Iwo maseru akaratidzirwa mushure mekuwedzera kwe monolayer uye kutevera 3D tsika nekuyerera cytometry, gene expression, uye histology.Kuchinja kwekutanga kwechiratidzo chekushandura nzira panguva yeredifferentiat ion yakaratidzwa.Masero eP2 akaratidza progenitor-like antigen profile ye 99% CD44 (+) uye 40% CD105 (+) uye gene expression profile inokurudzira masero e interzone.P2 muSF3D yakaratidza chondrogenic majini uye yakaunganidzwa extracellular matrix.Kuderedza insulini receptor (IR) neHNMPA- (AM3) kana PI-3 / AKT kinase nzira (yakagadzirwa ne insulini kurapwa) neWortmannin inhibited collagen synthesis.HNMPA-(AM3) yakaderedzwa kutaura kweCol2, Col11, uye IR majini pamwe neSox6 uye -9.Co-immunoprecipitation uye chromatin immunoprecipitation analyzes yeHNMPA-(AM3)-yakabatwa masero yakaratidza kusunga kweiyo coactivators Sox6 uye Med12 neSox9 asi yakaderedzwa Sox9-Col2a1 kusunga. hyaline-yakaita cartilage tissue formation muchikamu ne insulin-mediated Sox9-Col2a1 inosunga.Kukodzera kwematishu anogadzirwa nenzira iyi kuti ishandiswe mukugadziriswa kwemajoini kunoda kuongororwa muvivo.