L-Leucine Cas: 61-90-5 99% White powder
| Catalog Number | XD90304 |
| Product Name | L-Leucine |
| CAS | 61-90-5 |
| Molecular Formula | C6H13NO2 |
| Molecular Weight | 131.17292 |
| Storage Details | Ambient |
| Harmonised Tariff Code | 29224985 |
Chigadzirwa Specification
Zvakafanana nemamwe mavhairasi akanaka-strand RNA, tombusviruses inodzokororwa ne membrane-bound viral replicase complex (VRC).Iyo VRC ine p92 hutachiona-coded RNA-inotsamira RNA polymerase (RdRp), iyo hutachiona p33 RNA chaperone, uye akati wandei akasarudzwa mapuroteni ekugamuchira.Kuti ive inoshanda RdRp mushure mekushandurwa kwayo, iyo p92 replication protein inofanirwa kuiswa muVRC, ichiteverwa nekuita kwayo.Isu takamboratidza mune sero-isina mbiriso inobvisa-yakavakirwa assay kuti activation yeTomato bushy stunt virus (TBSV) RdRp inoda soluble host factor (s).Muchinyorwa chino, isu tinocherekedza cellular heat shock protein 70 (Hsp70) seyakabatana yakasarudzika chinhu chinodiwa kuti iite activation yeN-terminally truncated recombinant TBSV RdRp.Uye zvakare, diki-morekuru-yakavakirwa kuvharika kweHsp70 basa inodzivirira RNA synthesis neiyo tombusvirus RdRp in vitro.Uyezve, isu tinoratidza kuti kwaakarerekera phospholipids, kureva, phosphatidylethanolamine (PE) uye phosphatidylcholine (PC), inosimudzira RdRp activation in vitro.Mukupesana, phosphatidylglycerol (PG) inoratidza yakasimba uye ine simba inhibitory mhedzisiro pane in vitro RdRp activation.Isu tinoratidzawo kuti PE nePC zvinomutsa RdRp-viral plus-strand RNA [(+)RNA] kupindirana, ukuwo PG ichivharira kusungwa kwehutachiona RNA kuRdRp.Zvichienderana nekusimudzira kunopesana nemabasa ekudzivirira eakasiyana phospholipids mutombusvirus RdRp activation, isu tinokurudzira kuti lipid inoumbwa yeakatariswa subcellular membranes inogona kushandiswa netombusviruses kutonga mutsva VRC kusangana panguva yehutachiona. The virus-coded RNA-inotsamira RNA polymerase. (RdRp), iyo ine basa rekugadzira hutachiona hweRNA chizvarwa mumaseru ane hutachiona eanoverengeka akanaka-strand RNA mavhairasi, pakutanga haishande.Iri zano rinogona kudzivirira hutachiona hweRNA synthesis mucytosol iyo yaizokurumidza kutungamira mukuiswa kweRNA-yakakonzeresa cellular antiviral mhinduro.Munguva yekuungana kwe membrane-bound replicase complex, hutachiona RdRp hunoita activated kuburikidza nehurongwa husina kunzwisiswa hunoita kuti RdRp ikwanise kusanganisa RNA.Nekushandisa TBSV RdRp, isu tinoratidza kuti iyo yakasarudzika cellular Hsp70 chaperone uye nerere phospholipids inofambisa RdRp activation in vitro.Kusiyana neizvi, phosphatidylglycerol (PG) ine inhibitory mhedzisiro pane in vitro RdRp activation uye RdRp-viral RNA kupindirana, zvichiratidza kuti membranous microdomain yakatenderedza RdRp inokanganisa zvakanyanya kugona kwayo kweRNA synthesis.Nekudaro, iyo activation yehutachiona RdRp ingangove inotsamira pane akawanda anogamuchira zvikamu mumaseru ane hutachiona.
4-Nitrophenyl-N-acetyl-β- D-glucosaminide inobatsira substrate yekukurumidza colorimetric assay yeN-acetyl-b-glucosaminidase basa mumuguta wevanhu.Chromogenic β-Glucosaminidase substrate inobereka yero mhinduro pane cleavage, inoshandiswa kunyanya mumbiriso uye mold.
