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Kuunganidzwa kwenyukireya kweiyo mutant fomu yeprotein yenyukireya Lamin-A, inonzi Progerin (PG) kana Lamin AΔ50, inoitika muHutchinson-Gilford Progeria Syndrome (HGPS) kana Progeria, chirwere chinowedzera kuchembera.Chimwe chezviratidzo zvikuru zveiyi genetic disorder kurasikirwa kwe sub-cutaneous fat nekuda kwe dramatic lipodystrophy.We stably induced kutaura kwemunhu PG uye GFP -Green Fluorescent Protein- sekutonga mu3T3L1 masero uchishandisa lentiviral system kudzidza Mhedzisiro yePG kutaura mukusiyanisa kugona kweiyo sero mutsara, imwe yeanonyanya kushandiswa adipogenic modhi.Quantitative proteomics (iTRAQ) yakaitwa kuti iongorore mhedzisiro yePG kuunganidza.Akawanda emapuroteni akagadziriswa akasimbiswa ne immunoblotting uye chaiyo-nguva PCR.Basa reMitochondrial rakaongororwa nekuyera kwe) iyo mitochondrial basal chiitiko, b) iyo superoxide anion kugadzirwa uye c) kugona kwega kwega kwakasiyana kwakaoma kweketani yekufema. kuderera kwehuwandu hwekuwedzera uye kune hurema mune adipogenic kugona kana ichienzaniswa nekutonga.Quantitative proteomics analysis yakaratidza 181 mapuroteni zvakanyanya (p <0.05) yakagadziriswa muPG-inoratidza preadipocytes.Mitochondrial basa iri kukanganisa muPG-inoratidza maseru.Kunyanya, isu takaona kuwedzera kwekuita kweiyo yakaoma I uye kuwedzeredza kwe Superoxide anion.Incubation neReactive Oxygen Species (ROS) scavenger agents inotyaira kudzikira kweautophagic proteolysis sezvakaburitswa neLC3-II/LC3-I ratio.PG kutaura mu3T3L1 maseru kunosimudzira shanduko mune akati wandei Biological process, kusanganisira chimiro che cytoskeleton, lipid metabolism, calcium. mutemo, kududzira, kupeta mapuroteni uye kugadzirwa kwesimba nemitochondria.Yedu data inosimbisa mupiro weROS kuunganidza kune yekukurumidza kuchembera phenotype uye kumisikidza chinongedzo pakati pemitochondrial dysfunction uye kurasikirwa kweproteostasis muHGPS.