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Muedzo wakapfava wenzvimbo wakagadzirwa, unobvumira quantification ye microalbuminuria.Ongororo yakaitwa maererano ne ISO 15189 nhungamiro. Weti yakaonekwa pamitsetse ye cellulose acetate uye yakasvibiswa ichishandisa mapuroteni anosunga madhayi akasiyana-siyana (nigrosin, Coomassie Blue R-250, amido black).Kusimba kwemavara enzvimbo dzakasvibiswa kwakaverengerwa pachishandiswa chiteshi cheKodak Image 450. Analytical sensitivity yeCoomassie Blue based method (18 mg/L) yaiva nani pane iyo ye nigrosin (50 mg/ L) kana amido black (100 mg/L) ) yakavakirwa nzira.Mukati-run-run coefficient of variation (CV) uye pakati-run CV yeCoomassie blue assay yaive, zvichiteerana, 8.4% uye 9.7% (50 mg/L), uye 3% uye 4.5% (400 mg/L).Kune nigrosin, idzi data dzaive, zvichienderana, 8.4 uye 9.4 (50 mg/L), uye 3.4 uye 6.4% (400 mg/L).Coomassie Blue yakaratidza kusarudzika kunosunga kusarudzwa kune albumin.Nzira yacho yakawanikwa iine mutsara pakati pe20 ne600 mg / L.Kubatana kwakanaka (r2 = 0.89) kwakawanikwa pakati peCoomassie Blue yakavakirwa uye immunonephelometric zviyero.Immuno-unreactive albumin (yakagadzirirwa neprotease kurapwa) inogona kuwonekwa neiyo nzvimbo yekuyedza, iyo inopa mukana weiyo nzira kurwisa immunochemical bvunzo.Ammonium sulphate precipitation inogona kuwedzera kuwedzera kweiyo assay nekubvisa mhedzisiro yemaketani emwenje emahara.Nzira yakatsanangurwa iri nyore uye yakachipa zvakanyanya, izvo zvinoita kuti ive yakakodzera kutarisisa zvirongwa, kunyanya munyika dzechitatu.