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Acridine ester (NSP-SA-NHS) Cas199293-83-9 uye makomisheni ayo ane hukama anobatsira kwazvo chemiluminescent labels ayo kugadzikana, kuita uye kunzwa kunopfuura mamwe radioisotopes.Acridine esters inogona kuita nemapuroteni ane ekutanga amino mapoka.Pasi pemamiriro ezvinhu ekutanga, NHS inotsiviwa seboka rekubva, uye puroteni inoumba yakagadzikana amide bond neacridine ester.Mushure mekunge maitiro apera, iyo yakawandisa acridinium munyu yakabviswa kuburikidza nekoramu ye desalting.

Acridine-labeled mapuroteni haadi enzymatic catalysis kuti abudise chiedza pamberi pealkaline hydrogen peroxide.Iyo chaiyo yechiedza-emitting musimboti ndeyekuti mune alkaline hydrogen peroxide mhinduro, acridine ester inorwiswa nehydrogen peroxide ion kuti ibudise isina kugadzikana dioxyethane ine tension, iyo inowedzera yakaora kuita CO2 uye yemagetsi inonakidza acridone.Kana iyo acridone ichidzokera kumamiriro epasi, inoburitsa mafotoni ane yakanyanya absorption wavelength ye430 nm.Iyi luminescence process ipfupi kwazvo (yose iyo nzira inotora isingasviki 2 masekonzi), uye chirongwa chinokonzeresa chinofanira kuwedzera mukati photometer uye photon detector;uyezve, chigadzirwa ichi chinogona zvakare kushandisa yakawanda-inoshanda microplate muverengi akashongedzerwa neautosampler ye luminescence data collection.Mapuroteni, peptides, masoja ekudzivirira chirwere, uye nucleic acids zvese zvinogona kunyorwa nechigadzirwa ichi.Acridine esters inoburitsa chiedza nekukurumidza pasi pekunakidzwa kwealkaline hydrogen peroxide, saka iyo yakanyorwa makomisheni inogona kuonekwa nekuunganidza mafotoni.

Ichi chigadzirwa chinonyanya kushandiswa ku: chemiluminescence uye immunoassay, receptor kuongororwa, nucleic acid uye peptide yekuona uye kumwe kutsvagisa.