p-Nitrophenyl -aL-Fucopyranoside Cas:10231-84-2 Ichena kusvika kucheneruka yecrystalline poda
| Catalog Number | XD90142 |
| Product Name | p-Nitrophenyl -aL-Fucopyranoside |
| CAS | 10231-84-2 |
| Molecular Formula | C12H15NO7 |
| Molecular Weight | 285.25 |
| Storage Details | 2 kusvika ku8 °C |
| Harmonised Tariff Code | 29400000 |
Chigadzirwa Specification
| Chitarisiko | Upfu hwekristaro huchena kusvika kucheneruka |
| Assay | 99% |
| TLC | Single spot |
| Purity HPLC | Zvishoma 98% |
| Solubility (1% mumvura) | Yakajeka mhinduro isina ruvara |
| Density | 1.503±0.06 g/cm3 (20 ºC 760 Torr), |
| Melting point | 196-197 ºC |
| Boiling point | 515.4°Cat760mmHg |
| Flash point | 265.5°C |
| Refractive index | 1.623 |
LecA (PA-IL) inonzi cytotoxic lectin uye adhesin inogadzirwa nePseudomonas aeruginosa inosunga hydrophobic galactosides nehupamhi hwepamusoro uye hukama.Nekushandisa lecA-egfp dudziro fusion uye immunoblot ongororo yebiofilm extracellular matrix, tinoratidza kuti lecA inoratidzwa mumasero akakura ebiofilm.Mune static biofilm assays pazvese polystyrene nesimbi isina tsvina, kudzika kwebiofilm uye kufukidzwa kwepasi kwakaderedzwa nekuchinja kwelecA uye kukwidziridzwa muLecA-overproducing strain PAO-P47.Biofilm pamusoro pekuvharidzirwa nemubereki kunetsa, PAO-P47 asi kwete iyo lecA mutant pamakoni esimbi yakavharwawo nekukura pamberi peisopropyl-beta-D-thiogalactoside (IPTG) kana p-nitrophenyl-alpha-D-galactoside ( NPG).Uyezve, akakura emusango-mhando biofilms akaumbwa pasina aya hydrophobic galactosides anogona kuparadzirwa nekuwedzera kweIPTG.Kusiyana neizvi, kuwedzera kwep-nitrophenyl-alpha-L-fucose (NPF) iyo ine hukama hwepamusoro hweP. aeruginosa LecB (PA-IIL) lecti n yakanga isina simba pakuumbwa kwebiofilm kana kupararira.Planktonic kukura kweP. aeruginosa PAO1 haina kukanganiswa nekuvapo kweIPTG, NPG kana NPF, uye dambudziko racho harina kukwanisa kushandisa mashuga aya secarbon sources, zvichiratidza kuti zvakaonekwa pakuumbwa kwebiofilm zvakakonzerwa nekudzivisa kukwikwidza kweLecA-ligand. kusunga.Mibairo yakafanana yakawanikwawo kune biofilms inokura pasi pesimba rekuyerera kwesimbi makuponi, zvichiratidza kuti LecA inobatsira kuP. aeruginosa biofilm architecture pasi pemamiriro akasiyana ezvakatipoteredza.
An alpha-L-fucosidase (EC 3.2.1.51) inokwanisa kusunungura t-fucosyl yakasara kubva kumativi eketani ye xyloglucan oligosaccharides yakaonekwa mumashizha emiti yeArabidopsis.Zvakare, iyo alpha-L-fucosidase ine yakafanana substrate chaiyo yakacheneswa kubva mukabichi (Brassica oleracea) mashizha kuti ipe bhendi rimwechete paSDS-PEJI.Maviri epeptide akateedzana akawanikwa kubva muprotein band iyi, uye akashandiswa kuona Arabidopsis gene coding yealpha-fucosidase yatinoda kudaidza AtFXG1.Pamusoro pezvo, jini reArabidopsis rine homology ine inozivikanwa alpha-L-fucosidases yakawanikwa zvakare, uye isu takaronga kuitumidza kuti AtFUC1.Ose ari maviri AtFXG1 uye ATFUC1 akaratidzirwa zvine hutemo muPichia pastoris masero uye alpha-L-fucosidase zviitiko zvakavanzwa kune tsika svikiro.Iyo alpha-L-fucosidase yakavharidzirwa neAtFXG1 yakashanda ichipikisa oligosaccharides kubva ku xyloglucan XXFG pamwe chete ne 2'-fucosyl-lactitol asi kwete kupesana ne p-nitrophenyl-alpha-L-fucopyranoside.Nekudaro, iyo AtFUC1 yairatidzwa heterologously yaingoshanda chete 2'-fucosyl-lactitol.Nekudaro, iyo yekutanga inofanirwa kuve yakabatana ne xyloglucan metabolism.
